Compared with adults, levels of the repressor of HO-1 transcription, Bach1, were higher in the neonatal lung as was nuclear protein-DNA binding to the antioxidant response element (ARE) from HO-1. Furthermore, at baseline and in hyperoxia, chromatin immunoprecipitation (ChIP) revealed increased...
Heme oxygenase-1 (HO-1) protects cells from various insults including oxidative stress. Transcriptional activators, including the Nrf2/Maf heterodimer, have been the focus of studies on the inducible expression of ho-1. Here we show that a heme-binding factor, Bach1, is a critical physiological...
Knockdown studies using BACH1 siRNA and overexpression of miR-155 did not significantly rescue HO-1 expression in CFBE41o- cells. Elevated BACH1 expression detected in CF cells was refractory to the inhibitory function of miR-155 and was instead due to increased protein stability. Conclusion We...
Ferrostatin-1 (Fer-1), a potent inhibitor of ferroptosis, significantly alleviated DOX-induced cardiac damage. However, the cardioprotective effects of Bach1 knockdown were reversed by pre-treatment with Zinc Protoporphyrin (ZnPP), a selective inhibitor of heme oxygenase-1(HO-1). Taken together, ...
作为抗氧化基因的调控者,BACH1主要通过与抗氧化应答元件(AREs)结合,抑制下游基因如血红素加氧酶-1(heme oxygenase-1, HO-1)的表达。在正常状态下,这种抑制机制维持了细胞的氧化应激响应在低水平上的静息状态。然而,当氧化应激发生时,BACH1受到血红素(heme)结合的影响,被迅速移出细胞核,并通过SCFFBXO22和SCFFBXL...
作为抗氧化基因的调控者,BACH1主要通过与抗氧化应答元件(AREs)结合,抑制下游基因如血红素加氧酶-1(heme oxygenase-1, HO-1)的表达。在正常状态下,这种抑制机制维持了细胞的氧化应激响应在低水平上的静息状态。然而,当氧化应激发生时,BACH1受到血红素(heme)结合的影响,被迅速移出细胞核,并通过SCFFBXO22和SCFFBXL...
Bach1信号通路及其在皮肤病中的研究进展
HO-1 is a target of the oxidative stress-responsive transcription factor Nrf2. TGF-β did not affect the stabilization or nuclear accumulation of Nrf2 after stimulation with electrophiles. Instead, TGF-β induced expression of transcription factors MafK and Bach1. Enhanced expression of either MafK...
研究发现 Bach1 是一种亚 铁血红素结合蛋白,并且在血红素代谢中的主要降解酶——HO-1 的转录调控 中发挥着负性调控的作用[8]。换言之,高水平的亚铁血红素将抑制 Bach1 的 表达并因此上调 HO-1 的转录来达到抗氧化的作用[9]。Bach1 能在核内外出入, 核输出主要依靠输出蛋白 Crml,亚铁血红素能够抑制 Bach...