To determine the function of B7-H4 in LICs, we used B7-H4 knockout (KO) mice to establish an MLL-AF9-induced AML model and evaluated its role in the development of AML. As expected, the B7-H4 expression level was only detected on the wild-type (WT), but not B7-H4-null leukemia...
Notably, B7-H4 knockout hosts not only mounted greater tumor-associated anti-tumor T cell responses, but also displayed reduced tumors. Additionally, B7-H4-deficiency synergized with gemcitabine to further inhibit tumor growth, often leading to tumor eradication and the generation of protective T ...
Specifically, a cancer cell line (MDA-MB-468) was transplanted into MHC knockout NOG mice (Central institute for Experimental Animals), and after 2 weeks or later the Fab-scFv according to the present invention labelled with the fluorescent dye Cy5.5 was administered in a range of 0.5 microgr...
B7-H4-/-pregnant mice (pregnant mice with B7-H4 gene knockout) and purified primary human dMφ treated with B7-H4 neutralizingantibody were used to explore the role of B7-H4 signaling on regulating the membrane molecules, synthesis of arginine metabolic enzymes and cytokine production by dMφ ...