b-Galactosidase activity assay using far-red-shifted fluorescent substrate DDAOGGongH.ZhangB.LittleG.KovarJ.ChenXieingentaconnectAnalytical BiochemistryGong H, Zhang B, Little G, Kovar J, Chen H, Xie W, Geschwender AS, Olive DM (2009). β-galactosidase activity assay using far-red-shifted ...
The basic colorimetric assay described here is the simplest and least expensive assay for quantifying beta-gal activity. The cells are lysed and, after determining the total protein concentration in the extracts, an aliquot of the extract is mixed with the reaction substrate, O-nitrophenyl-beta-D...
Tumor homogenates and blood serum samples were then analyzed by b-galactosidase assay. The results revealed high enzyme activity in tumor samples (B100 U gÀ1 tumor tissue), but undetectable levels in blood serum samples (detection limit: 0.3 U mlÀ1). Therefore, un- wanted side effects ...
The Src tyrosine-kinase SmTK3 is involved in processes which control mitotic activity and differentiation in Schistosoma mansoni. Using the yeast two-hybrid system it was possible to identify several interaction partners of SmTK3, and their relative interaction strength were determined via a b-...
Anti-HIV-1 activity determined by β-galactosidase activity in the multinuclear activation of an indicator assay is comparable with that by a conventional ... Anti-HIV-1 activity determined by β-galactosidase activity in the multinuclear activation of an indicator assay is comparable with that by ...
suis BgaC was proved to obviously possess the activity of b-galactosidase with Km of 0.398 mM, Kcat of 5.63 3 102/s and Vm7.43 3 1023 mM/min (Fig. 2C). It seemed likely that our structure-guided prediction in this case is correct (Fig. 1). Linkage specificity of S. suis BgaC ...
coli cells by transformation; and 3) selection of ampicillin resistant transformants; selection and growth of Lac+ and Lac- colonies; assay of these colonies for β-galactosidase activity. As an optional activity, the recombinant plasmids may be extracted from cells, digested with restriction ...
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A small library of 22 N-substituted galactonoamidines was synthesized, and their structure-activity relationship for inhibition of the hydrolytic activity of β-galactosidase (Aspergillus oryzae) was evaluated. A fast screening assay in 96-well plate format was used to follow the enzymatic hydrolysis...
Aging of human liver cell lines is accompanied by a decrease in the lysosomal β-D-galactosidase activity. In six cell lines, both catalytic assay using 4-methylumbelliferyl-β-D-galactopyranoside and immunochemical titration by a monospecific anti-β-D-galactosidase anti-serum were performed. The ...