The invention is directed to an assay for acid 尾-galactosidase activity. The invention may include combining in oil a sample droplet with a 4-methylumbelliferyl-B-galactose to yield a reaction droplet; splitting the reaction droplet to yield a first daughter droplet and a second daughter droplet...
b-Galactosidase activity assay using far-red-shifted fluorescent substrate DDAOGGong, H.Zhang, B.Little, G.Kovar, J.Chen, H.Xie, W.Schutz-Geschwender, A.Olive, D. M.ANALYTICAL BIOCHEMISTRYGong H, Zhang B, Little G, Kovar J, Chen H, Xie W, Geschwender AS, Olive DM (2009). β-...
Tumor homogenates and blood serum samples were then analyzed by b-galactosidase assay. The results revealed high enzyme activity in tumor samples (B100 U gÀ1 tumor tissue), but undetectable levels in blood serum samples (detection limit: 0.3 U mlÀ1). Therefore, un- wanted side effects ...
suis BgaC was proved to obviously possess the activity of b-galactosidase with Km of 0.398 mM, Kcat of 5.63 3 102/s and Vm7.43 3 1023 mM/min (Fig. 2C). It seemed likely that our structure-guided prediction in this case is correct (Fig. 1). Linkage specificity of S. suis BgaC ...
coli cells by transformation; and 3) selection of ampicillin resistant transformants; selection and growth of Lac+ and Lac- colonies; assay of these colonies for β-galactosidase activity. As an optional activity, the recombinant plasmids may be extracted from cells, digested with restriction ...
Synthesis of novel NBD-GM1 and NBD-GM2 for the transfer activity of GM2-activator protein by a FRET-based assay system The ganglioside-activator protein is an essential cofactor for the lysosomal degradation of ganglioside GM2 (GM2) by beta-hexosaminidase A. It mediates the... S Günter,W...
A homogeneous enzyme immunoassay method for proteins is described. The assay components include a protein antigen—β-galactosidase conjugate, antiprotein antibody, and a synthetic macromolecular substrate. When antibody binds to the conjugate, enzyme activity is inhibited up to 95% because of steric ex...
contains an enzyme produced by aspergillus oryzae.itisanenzymedrug having lactose decomposition activity. it contains 8000 to 12000 units per g. usually, it is diluted with a mixture of maltose hy- drate and dextrin, maltose hydrate and d -mannitol, or maltose hydrate, dextrin and d -mannitol...
A small library of 22 N-substituted galactonoamidines was synthesized, and their structure-activity relationship for inhibition of the hydrolytic activity of β-galactosidase (Aspergillus oryzae) was evaluated. A fast screening assay in 96-well plate format was used to follow the enzymatic hydrolysis...
Previous experiments measuring β-galactosidase (β-gal) activity using a colourimetric enzyme activity assay have shown that the addition of lactose to cultures induced with isopropylthiogalactoside caused an apparent decrease in induct... N Dacosta,S Fowler,P Germann,... 被引量: 0发表: 2002年...