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The level of the β1-AR autoantibody was measured using ELISA kit (CUSABIO, Wuhan, China). An optical density (OD) sample/OD negative ratio < 2.1 was negative, while an OD sample/OD negative ratio ≥ 2.1was positive (if OD negative < 0.1, we calcu- lated it as 0.1)....
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q ELISA for the binding of Nogo-B to proGCG. r Non-linear fit analysis of ELISA results (n = 6 biological replicates). s Affinity constants of Nogo-B and proGCG detected by SPR. All experiments of (b–d, f–I, k, l, n–p) were repeated at least three times Data are ...
After washing of the immunoprecipitated complexes, DNA was eluted and purified with QIAquick PCR Purification kit (QIAGEN). The Re-ChIP assay was performed according to the established protocol94. Cells were subjected to crosslinking and chromatin shearing through sonication. The first antibody was ...
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