All 13 locals of Carlsbad finally took a tour of the caves, which came to be known as Carlsbad Caverns.”可知,Jim在1901年就发现了Carlsbad Cavens,但人们并不相信他,直到十几年后人们看 到了照片才相信了Carlsbad Caverns的存在,由此可知,是这些照片促成了人们对Carlsbad Caverns的认可。 4.B主旨大意...
A. Isle Royale National Park.B. Dry Tortugas National Park.C. Channel Islands National Park.D. Carlsbad Caverns National Park. 5B阅读理解墨尔本海洋生物水族馆The all-new Sea Life Melbourne Aquarium, situated in the heart of Melbourne's CBD, is one of Victoria's leadingvisitor attractions and ...
Myristoylation, the N-terminal modification of proteins with the fatty acid myristate, is critical for membrane targeting and cell signaling. Because cancer cells often have increased N-myristoyltransferase (NMT) expression, NMTs were proposed as anti-ca
Real-time PCR was carried out on the StepOnePlus Real-time PCR System (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) with SYBR Premix Ex Taq (TaKaRa). The sequences of primers were: miR-200b forward: 5′-TGCCGTAATACTGCCTGGTAA-3′, reverse: 5′-CAGAGCAGGGTCCGAGGTA-3′;...
She designe d an d made dolls for them.Fresh Start Surgical Gifts in Carlsbad. The 19-year-ol d organization provides C. She gave them some medical support free surgeries an d other medical treatment for children whose families can't D. She bought many gifts for them.affor d it. 26....
One hundred and fifty nanograms of total RNA from each PB and BM sample were reverse transcribed in a 20 µL reaction following the manufacturer’s specifications (Invitrogen, Carlsbad, CA, USA). The relative expression of 26 DEGs was performed by real-time quantitative PCR (RT-qPCR) using...
The forward primer, CVEV, was then used in a 3' rapid amplification of cDNA ends (RACE) reaction following the manufacturer's instructions (Invitrogen, Carlsbad, CA). A nested primer, DTEA (5'-GACACAGAGGCAGTGGCGG-3') was used for a second round of amplification, resulting in the ...
Genomic RNA was isolated using a commercial reagent kit (RNeasy Mini Kit; Qiagen, Hilden, Germany). Reverse transcription was conducted with the commercial kit (High-Capacity cDNA Reverse Transcription Kit; Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s guidelines. The ...
Total cellular RNA was extracted with RNeasy (Qiagen, Hilden, Germany) and converted into cDNAs by reverse transcriptase (Superscript II, Invitrogen, Carlsbad, CA). Real-time PCR was performed using the ABI PRISM 7000 Sequence Detection System (Applied Biosystems, Foster City, CA) according to ...
Larvae were dissected and their midguts removed for DNA extraction using the PowerSoil DNA Isolation Kit (MoBio, Carlsbad, CA). DNA integrity and concentration were verified using a NanoDrop spectrophotometer (Thermo-Fisher, Pittsburgh, PA) and the Quant-It dsDNA assay (Life Technologies, Carlsbad,...