细胞衰老检测试剂盒(B-半乳糖苷酶) PRODUCT CENTER 细胞分析试剂盒 当前位置:首页>产品中心>细胞生物学产品>细胞分析试剂盒
产品名称 细胞衰老检测试剂盒 / Cell Senescence β-Galactosidase Staining Kit 产品规格 1000T 型号 盒 用途范围 科研使用 是否进口 否 加工定制 否 包装规格 1kg 有效期 1年 是否现货 现货 最低订购 1盒 适用样本 1.悬浮细胞<br>2.贴壁细胞 应用 细胞衰老检测 品牌 BestBio贝博 价格说明 价格...
1)不可逆的生长停滞,细胞体积变大; 2)与衰老相关的β-半乳糖苷酶(senescence associated β-galactosidase, SA-β-gal)的活化。 作为溶酶体内的水解酶,通常在pH 4.0的条件下表现活性。 但是衰老细胞内SA-β-gal在pH 6.0的条件下表现活性,且随...
we hypothesized that adipocyte-secreted factors may induce cellular senescence in human B-ALL cells. To test this hypothesis, we cultured human B-ALL cells in unconditioned, SCM, or ACM for 24 h and measured β-Galactosidase (β-Gal
Senescence-associated beta-galactosidase assay Cell senescence was determined using the senescence β-galactosidase (SA-β-gal) staining kit (Cell Signaling) following the manufacturer’s instructions. At 24, 48, and 72 h post-transfection of SIRT4 plasmid, cells were fixed with a fixative soluti...
Senescence-Associated Beta-Galactosidase Marker of Cellular Senescence - ScienceDirectManjari DimriGoberdhan P. DimriEncyclopedia of Biomedical Gerontology
SA-β-gal assay We measured senescence-associated β-galactosidase (“SA-β-gal”) using a SPiDER-β-Gal detection kit (#SG-03, Dojindo, Kumamoto, Japan) according to the manufacturer’s protocol. Organoids seeded and cultured in a 48-well plate were immuno-labeled after ExM removal and ...
的BV2细胞活力的影响，并筛选出最佳的天麻素浓度；使用β-半乳糖苷酶（Senescence β-Galactosidase ，SA-β- Gal ）染色检测细胞衰老面积；使用生化法检测各组细胞活性氧（Reactive oxygen species ，ROS ）水平；使用 Enzyme linked immunosorbent assay （ELISA ）法检测各组细胞神经炎症因子IL-1β（Interleukin 1β...
Cdkn2b inactivation is necessary to make cell encompass senescence induced by oncogenic KRAS. Obvious senescence-associated β-galactosidase (SA-β-GAL) activity was detected in cells infected by KRAS-shTp53, KRAS-shTp53-shCdkn2a, KRAS-shTp53-shCdkn2b, but that was not detected in infected ...
Oncogenic B-RAF-V600E expressed in normal NCM460 colonocytes induced a senescent phenotype as evidenced by expression of senescence-associated β-galactosidase (SA-β-gal), the cell cycle inhibitors p14ARF, p15INK4B and p21WAF1, and decreased proliferation. Upon coexpression of RAC1B, but not...