In order to study tumor infiltrating B cells (TIBs), the tumor first has to be dissociated into a single cell suspension. For this, the gentleMACS™ Dissociator in combination with the Tumor Dissociation Kit, human ensures rapid and standardized dissociation of tissue samples into single-cell su...
产品名称:B Cell Expansion Kit, human .0订购此产品 供应商:miltenyi 规格:*** 目录价:56241.9 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址:点击查看详细 打印此页关闭此页 上一个:StemMACS HSC Expansion Cocktail[130-100-843/***] ...
130-093-617美天旎磁珠分选产品开关记忆B细胞Iso试剂盒,人130-093-619 IgM + Memory B Cell Isolation Kit, human130-093-626 CD197 (CCR7) pure, human130-093-627 T Cell Activation/Expansion Kit, mouse130-093-628 Plasma Cell Isolation Kit II, human咨询热线:18221103493(微信同号)品牌:miltenyi 美...
B cell:T cell co-culture Beff and B10 cells were obtained as mentioned above. CD4+CD25− naïve T cells from gd10 WT and µMT mice were isolated using a Treg isolation kit (Miltenyi Biotec,). 1 × 105 T cells were cultured with 1 × 105 Beff or B10 cells in anti...
T cells were enriched with the Human Pan-T Cell Negative Isolation kit (Miltenyi Biotec). Real-time PCR Total RNA was purified using TRIzol reagent (Invitrogen). cDNA synthesis was performed using SuperScript II Reverse Transcriptase (Invitrogen) after DNase I treatment (Invitrogen), and real-...
Naive splenic B cells were purified using the B cell isolation kit (Miltenyi Biotech) according to the manufacturer’s protocol. Purified B cells (purity 90%–92%) were then incubated either alone, with different ratios of parasites, or with TLR agonists: 3 μg/ml CpG (InvivoGen), 5 μ...
B cells were purified from whole spleen by negative selection with a MACS B cell isolation kit (Miltenyi Biotec, catalog no. 130-090-862) on MACS separation columns (Miltenyi Biotec, catalog no. 130-042-401) according to the instructions of the manufacturer. Fractionated B cells were >85% ...
Cell isolation Peripheral blood mononuclear cells (PBMCs) were isolated by standard Ficoll-Hypaque procedures. CD19+ B cells and CD4+ Naïve T cells were both enriched by negative selection with isolation kits (Miltenyi Biotec). The purity of B cells or Naïve T cells was determined by flow...
(Sigma Aldrich) and washed two times. Four samples were processed at the same time, randomizing RA samples with healthy controls. B cells were isolated by negative separation using the human B-cell isolation kit II (Miltenyi Biotec) and QuadroMACs separator. B cells were counted (yields varied...
Total B cells were enriched from mouse BM and spleen by using a MACSxpress B cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The purity of the enriched B cells was > 90% as determined by FACS after B220 staining. Mouse pre-B cells were further sorted by using a FACS...