6.Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mixandincubate for 15 minutes at37°C.Protect from light. 7.Add 50μlStop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or ...
In fact, many B chromosomes are known to harbor rRNA genes (Green, 1990). On A chromosomes, rRNA genes exist in tandem arrays localized at the nucleolar organizer regions (NORs). The presence of these genes on B chromosomes has been mainly demonstrated by silver impregnation, which detects ac...
those plants nearest the windows would be exposed to more sunlight and grow more quickly, reducing the efficiency of the system. Single-storey greenhouses have the benefit of natural overhead light: even so, many still need artificial lighting. A multi-storey facility with...
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Finally, real-time quantitative PCR (RT-qPCR) was carried out using the SYBR Green qPCR Master Mix (Applied Biosystems). mRNA expression values were calculated using the ΔΔCt method and GAPDH gene as a control. A detailed list with primers used in the present study is provided in Table ...
We need a lot of oil, and we are using up the oil wells we know about. Soon we must find new ways of looking for this black gold. 8 (1)、From this passage, we can know oil is a source of energy. A :T B :F 答案: A (2) 、According to the passage, we can tell that ...
I believe they’re looking at it through puce-colored glasses. Even as I choose to see through rose-colored glasses, I’m not blind to how poverty has ravaged many North County municipalities. Look at the corpses of Kinloch or Wellston to see the ways racism works through legal and ...
On the other hand, the French man may describe his ideal woman as a woman who stands only ive eet three inches with brown hair and green eyes (1) . Which o the ollowing will an “outdoor man “love to do according to the passage? 答案 (C) A. He will never ask an inside ...
1× PBS assay buffer. Sera were assayed at 1:500 dilution and surveyed for antibodies against nucleocapsid protein or RBD. After a 1-h incubation in the dark on a plate shaker at 800 rpm, wells were washed five times in 100 μl of assay buffer using a BioTek 405 TS plate washer...