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by the manufacturer (TaqMan Universal PCR Kit; Applied Biosystems). Primers and probes were from Applied Biosystems:Il12a(ABI, Mm00434169_m1),Ebi3(ABI, Mm00469264_g),Il10(ABI, Mm00439616_m1),gapdh(ABI, Mm99999915_g1),actin b(Mm00469264_g1) (Supplementary Table1). The mRNA expression le...
点击这里 填写评论 选择获取 Cruz Credit™ 优惠券 快捷链接 订购信息 描述 技术信息 安全信息 安全技术说明书和分析证明书描述技术信息安全信息安全技术说明书和分析证明书 埃博霉素B是一种16元大环内酯,具有微管稳定特性。研究表明,埃博霉素B在体外诱导微管蛋白聚合成微管。其他实验表明,埃博霉素B导致细胞周期在G...
Western blotting The following primary Abs were used: anti-CD49b, anti-STAT-1, anti-pSTAT-1 (Cell Signalling Technology Inc., Danvers, MA, USA), anti-CX3CL1 (eBioscience, San Diego, CA, USA), anti-CX3CR1 (eBioscience), anti-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA)...
(A3D) in a context-dependent manner. This result prompted us to hypothesize that A3B might also be linked to R-loops in our scenario. We tested this by IP-MS analysis with a monoclonal antibody (S9.6) to investigate R-loop interacting components. R-loops interact with Replication protein ...
Beta-Actin 抗体, 兔多抗, 抗原亲和纯化WB IHC-P IP 50 μg 现货供应 蛋白G凝胶5 mL 现货供应 重组大消化链球菌蛋白L (Protein L)5 mg 蛋白A凝胶50 mL 现货供应 Anti-DYKDDDDK Affinity Resin50 mL 现货供应 Protein L Agarose Beads / Resin5 mL 现货供应 ...
The actin expression, as an internal control of protein loading, was detected by mouse monoclonal antibody (Santa Cruz). ER Stress Signals on Hepatocytes in Liver Tissue To analyze the ER stress, the expression of GRP78 was performed on the LCM-harvested type I and type II GGHs and HBs-...
Forty-eight hours after transfection, the nuclear fraction was extracted and immunoprecipitated with monoclonal anti-Myc tag antibody. Immunoprecipitates were then Western blotted with anti-Myc (detecting recombinant CD40), anti–c-Rel, anti–Oct-1, and anti–β-actin antibodies. Input indicates 25 ...
About 200 μg anti-Pcsk9 monoclonal antibodies (alirocumab) were intraperitoneally injected on days 3, 5, 8, and 11. After 2 weeks, the pancreas was weighed (n = 6 mice). Three biologically independent experiments were performed (e...
The PureProteome Protein A/G Mix Magnetic Bead suspension (Merckmillipore, Darmstadt, Germany) was first incubated with an anti-ACVR2 polyclonal antibody (H-65, sc-25451, 0.5 µg/ml, Santa Cruz Biotechnology, CA, USA), with an anti-CD44 monoclonal antibody (clone IM7, #550538, 0....