Strikingly, multiple dynein arm assembly factors show structural similarities to either Tah1 or Pih1, the other two components of the R2TP complex. Based on these results, we propose that Pontin and Reptin function to facilitate dynein arm assembly in cytosolic foci enriched with R2TP-like...
Present understanding of the sequential steps in axonemal dynein assembly rests largely on genetic studies in Chlamydomonas, a single-celled alga that swims by beating its two flagella in a highly asymmetric, planar, ciliary style. Because this motility is nonessential for growth, dozens of motilit...
et al. CCDC39 is required for assembly of inner dynein arms and the dynein regulatory complex and for normal ciliary motility in humans and dogs. Nat. Genet. 43, 72–78 (2011). CAS PubMed Google Scholar Becker-Heck, A. et al. The coiled-coil domain containing protein CCDC40 is ...
The Chlamydomonas reinhardtii ODA3 Gene Encodes a Protein of the Outer Dynein Arm Docking Complex We have used an insertional mutagenesis/ gene tagging technique to generate new Chlamydomonas reinhardtii mutants that are defective in assembly of the out... Koutoulis,A. - 《Journal of Cell Biology...
Taken together these data suggest that all three of the intermediate chains in the I1 dynein complex play key roles in the assembly, anchoring and regulation of the complex. This study tested the hypothesis that IC140 and IC138 can bind directly to tubulin and thus are involved in anchoring ...
Cooperative assembly through inter-dynein contacts The three AAA+ domains of the ODA are stacked in parallel, with the motor domain of the γ-HC closest to the doublet microtubule surface but not in contact with it, and α-HC furthest away (Fig. 1). Stripping the ODA complex from double...
mutation of Rsph2339might disrupt the head-neck assembly by truncating the protein (Fig.2b). The I139Yfs*8 mutation of Rsph23 truncate its C-terminus40, which is responsible for its binding to Rsph16 to stabilize the head-neck complex, and may therefore induce instability of the complex....
CCDC39 is required for assembly of inner dynein arms and the dynein regulatory complex and for normal ciliary motility in humans and dogs. We exploited the unique properties of dog populations to positionally clone a new PCD gene, CCDC39. We found that loss-of-function mutations in the ......
Primary ciliary dyskinesia most often arises from loss of the dynein motors that power ciliary beating. Here we show that DNAAF3 (also known as PF22), a previously uncharacterized protein, is essential for the preassembly of dyneins into complexes before their transport into cilia. We ...
dynein arm assemblydynein armsmotile ciliaThe microtubular scaffold of motile cilia-the axoneme, is decorated with dynein arms, which are large multiprotein complexes essential for ciliary motility. Dynein arms are arranged along the length of the axoneme in a preciserepeating pattern, converting ...