1 Insert the key on the back of the Axon Body 2 camera into the lock. 2 Twist the Axon RapidLock mount counterclockwise, 90 degrees. 3 Place the magnetic back underneath the shirt, patrol vest, or whatever you are using. 4 Hold the back in place, and place the Axon RapidLock mount ...
After mRNA purification using oligonucleotide (dT)-attached magnetic beads, cDNA fragments were generated by reverse transcription with random hexamer primers and amplified by polymerase chain reaction (PCR). After the reaction was purified with Ampure XP Beads, it was dissolved in ethidium bromide ...
aSchematic protocol for embryoid body (EB) based neuronal differentiation using 46C embryonic stem cells (ESC). Differentiation medium included Glasgow’s modification of Eagle’s Minimal Essential Medium (GMEM) and Knockout Serum Replacement (KSR) for EB day 0 to day 8 while retinoic acid (RA)...
functions in its own right. Notably, 5hmC is highly abundant in the CNS, accounting for ~40% of modified cytosines, mostly in gene body regions19,20,21,22, and is pivotal for neuronal differentiation22,23. While these earlier studies showed the necessity of Tet3 and DNA demethylation for ...