For the preparation of single-cell ATAC-seq libraries, we employed the DNBelab C Series Single-Cell ATAC Library Prep Set (MGI, #1000021878)19. To investigate the transcriptional regulation of different brain regions in axolotl with sufficient and high-quality data, a total of 39 libraries were ...
processes such as DNA replication, mitosis, and cell cycle are enriched by a set of up-regulated genes the majority of which signify a transition phase in the limb regeneration program
The qRT-PCR was carried out using miScript SYBR Green PCR kit (Qiagen) per manufactures protocol. Either Qiagen designed primers compatible with miScript kit were purchased to quantify conserved microRNAs, and custom designed primers were made by IDT to amplify axolotl mRNAs. Custom axolotl ...
The resulting PCR product was used to synthesize in situ probe by the addition of DIG-labeled UTP (Roche) plus the appropriate RNA Polymyerase T7 or Sp6 (NEB). Probes were purified with RNA Clean Up kit (Qiagen) and resuspended in 100 μL of hybridization buffer. Primers for other g...