Previous studies have shown that some other cell lines can form colonies under arginine deprivation, such as in MCF-7 cells with a 50% knockdown of ASS1 [24] and BxPC-3 and MIA PaCa-2 cells grown in glutamine-free media [25]. However, in our colony formation assay, we observed that ...
TUBO and MCF7 cells in serum-free DMEM-F12 (Thermo Fisher Scientific) supplemented with 20 ng/ml epidermal growth factor (cat. no. 315-09; Peprotech), 20 ng/ml basal fibroblast grow factor (cat. no. 100-18; PeproTech), 5 μg/ml insulin (cat. no. 19278; Merck) and 0,4%...
express CCL2 at high levels (Rego et al.2013; Yoshimura et al.2013). Through the activation of Smad3 and p42/44 MAPK signaling, CCL2 was found to boost the migration and survival of 4T1, PyVmT, MDA-MD-231, and MCF-7 cells as well as...
MCF7 EDR and MCF7 TamR cells were exposed to increasing concentrations of F+P to generate R derivatives (FPR, cells grow in the presence of F 100nM + P 100nM). The transcriptomic and proteomic profiles of MCF7 EDR, TamR, EDR/FPR and TamR/FPR were determined by RNA-seq and reverse-...
induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. J Ethnopharmacol. 2012;141(3):803–809. doi:10.1016/j.jep.2012.03.014 21. Balamurugan R, Stalin A, Ignacimuthu S. Molecular docking of gamma-sitosterol with some targets related to diabetes...
induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. J Ethnopharmacol. 2012;141(3):803–809. doi:10.1016/j.jep.2012.03.014 21. Balamurugan R, Stalin A, Ignacimuthu S. Molecular docking of gamma-sitosterol with some targets related to diabetes...
MDA-MB-231, JIMT1, SKBR3 and MCF7 cells were seeded and allowed to grow to 50-60% confluency for 24 h prior to transfection with control siRNA and siRNA targeting c-MYC using Lipofectamine RNAi-MAX Reagent. siRNA used included MYC siRNA (VHS40785; Cat. No. 1299001), FTH1 siRNA (s22...
Human: MCF7 breast cancer cell line: TP53 WT Stanford University School of Medicine laboratories N/A Human: MDA-MB-231 breast cancer cell line: TP53 MUT Stanford University School of Medicine laboratories N/A Experimental Models: Organisms/Strains Mouse: Trp53flox mice: B6.129P2-Trp53tm1Brn/J...
UponRPSAP52knockdown, all three breast cell lines tested (the non-transformed MCF10A and the tumorigenic Hs578T and HCC1143 cells) proved to be significantly less proliferative in the sulforhodamine B (SRB) assay (Fig.3a), and had a significantly lower percentage colony formation density than ...
The results designated that circTRIM28 was constrained in MCF7/R and MDA-MB-231/R cells by sh-circTRIM28 (Fig. 2A). Among them, sh-circTRIM28#1 had the most obvious inhibiting effect, so it was selected for subsequent tests. Function- ally, the downregulated circTRIM28 diminished ...