m1004, M1004-W, M1013, M1025, M1034, M1034-W, M1051, m1054, M1054, M1065-L, M1065-LW, m1113, M1114, M1124, M2025, M2025-LE, M2026-LE Mk II, M2026-LE mk2, M3004, M3004V, M3005, M3005-V, M3006, M3006-V, M3006-V D
MK2-mediated phosphorylation of MRTF-A at Serand Serwas further confirmed in ankinase assay and using the phospho-protein kinase-D (PKD)-consensus motif antibody (anti-LXRXXpS/pT), the p38inhibitor BIRB-796, MK2/3-deficient cells and MRTF-A phospho-site mutants. Unexpectedly, dimerization, ...
48 Other kinases besides P-TEFb might contribute to NELF regulation in vivo; ERK has been reported to phosphorylate NELF-A to promote the transcription of immediate-early genes,116 and MK2 phosphorylates NELF-E in response to UV-induced DNA damage.117 In response to stress, NELF is de...
SeV was propagated in specific pathogen-free 10-days embryonated chicken eggs (Charles River Laboratories) and titered on LLCMK2 cells (75) and SeV infection was performed as described (16). Single-cycle HIV-1 luciferase pseudotyped with VSV-G (HIV-1-Luc/VSV-G) was generated in HEK293...
m1004, M1004-W, M1013, M1025, M1034, M1034-W, M1051, m1054, M1054, M1065-L, M1065-LW, m1113, M1114, M1124, M2025, M2025-LE, M2026-LE Mk II, M2026-LE mk2, M3004, M3004V, M3005, M3005-V, M3006, M3006-V, M3006-V Dome, m3007, M3007, M3014, M3024-L, m...
Mechanistically, we show that MK2 phosphorylates the RNA-binding subunit of the NELF complex NELFE on Serine 115. NELFE phosphorylation promotes the recruitment of 14-3-3 and rapid dissociation of the NELF complex from chromatin, which is accompanied by RNA polymerase II elongation. UV-light-...