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Fluorescence intensities of GFP and mNeonGreen were measured using Fiji/ImageJ84,85. For the polymerase subunits degradation experiments shown in Extended Data Figs. 5a–f and 7a, for each strain, images of the different treatments were acquired with the same settings. Intestinal nuclei were selec...
The band intensity of each protein after immunoblotting was quantified using the FIJI software51. To derive Rad9 levels, the Rad9 band signal in each lane was first normalized to the loading control (β-Actin). The small variations of Rad9 band intensities among different immunoblots stemmed ...
The fluorescence intensity and autophagolysosomal puncta were analyzed with Fiji software. Flow cytometry analysis Y79 and WERI-Rb-1 cells from the control and treatment groups were collected and washed twice with phosphate-buffered saline (PBS). For the cell cycle analysis, the cells were fixed ...
The band intensity of each protein after immunoblotting was quantified using the FIJI software51. To derive Rad9 levels, the Rad9 band signal in each lane was first normalized to the loading control (β-Actin). The small variations of Rad9 band intensities among different immunoblots stemmed ...
Colocalization was estimated using the Colocalization plug-in of Fiji software (Image J version 1.54 m, National Institutes of Health, MD, USA). Overlap between the expression protein was quantified using Pearson’s colocalization coefficients. Transmission electron microscopy(TEM) The cells were ...
Western blot quantifications were performed using FIJI ImageJ version 1.53q41 (https://imagej.net/software/fiji/downloads; RRID: SCR_002285). The signal intensity for each protein band of interest analyzed was normalized to its corresponding loading control. Fold change was calculated related to ...
To avoid potential human subjectivity in imaging analysis, a Macro program on ImageJ/Fiji automating the quantification of mitophagy as indicated by Mito-pHfluorin was generated. Procedures for quantification of the red puncta are outlined in Fig. S2 using the following computer programme: if (isOp...
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To avoid potential human subjectivity in imaging analysis, a Macro program on ImageJ/Fiji automating the quantification of mitophagy as indicated by Mito-pHfluorin was generated. Procedures for quantification of the red puncta are outlined in Fig. S2 using the following computer programme: if (isOp...