黑龙江省稻瘟病菌无毒基因Avrpiz-t群体结构分析
Avrpiz—t基因序列设计特异引物,对黑龙江省四个作物种植区的108个稻瘟病茵茵 株进行PcR扩增,明确稻瘟病菌无毒基因Avrpiz—t在黑龙江省四个作物种植区的分布情况。结果表明,供试108个 菌株中有82%的菌株是正常基因,广泛分布于黑龙江省四个作物种植区,在供试的菌株中10.4%的菌株未扩增出条 ...
To demonstrate the translocation of AvrPiz-t into host cells, we focused on three independent trans- formants that showed bright fluorescence of AvrPiz-t:mCherry: NLS. At successful infection sites where BAS4:EGFP was not spilled into host cells (n = 22) (Figure 1, EGFP, bottom panel), ...
The Magnaporthe oryzae avirulence gene AvrPiz-t activates immunity in a gene-for-gene fashion to rice mediated by the blast resistance gene Piz-t. To dissect the molecular mechanism underlying their recognition, we initiated the cloning of AvrPiz-t using a map-based cloning strategy. The AvrPiz-...
病原菌分泌的效应子对宿主的侵染至关重要。虽然许多效应子在不同的病原菌中已被鉴定,但其结构域或氨基酸序列在效应子功能发挥过程中的作用知之甚少。本文探究了稻瘟病菌效应子AvrPiz-t的赖氨酸残基在其介导的水稻抗性(ETI)和基础防御(PTI)过程中的作用机制。
The rice blast resistance gene Piz-t mediates resistance to the devastating fungal disease rice blast by recognizing its cognate avirulence gene AvrPiz-t in M. oryzae. A total of 12 AvrPiz-t interacting proteins, designated as APIPs, were identified by a yeast-two-hybrid approach. APIP12 showe...
The rice blast resistance gene Piz-t mediates resistance to the devastating fungal disease rice blast by recognizing its cognate avirulence gene AvrPiz-t in Magnaporthe oryzae.A total of 12 AvrPiz-t interacting proteins,designated as APIPs,were identified by a yeast-two-hybrid approach. APIP12 enc...