The present invention relates to an isolated protein which is structurally and functionally avidin-like with improved properties, such as better affinity towards biotin conjugate, useful immunological properties or faster biotin dissociation rate, compared to avidin and streptavidin. Additionally, it relates...
Two avidin-like proteins, Tamavidin 1 and Tamavidin 2, were previously isolated from the Tamogitake mushroom (28), and one point mutation (S36A) was introduced into Tamavidin 2 to break the hydrogen bond between Tamavidin 2 and biotin (29). This engineered Tamavidin 2, named Tamavidin ...
Embodiments described herein relate to methods for reversible binding of a biotin-binding protein, e.g., rhizavidin, including fusion proteins and complexes thereof to a matrix comprising a lipoic acid (LA) compound or derivative thereof immobilized to a solid support, where the biotin-binding ...
Avidin-like proteins from symbiotic bacteriaAn isolated protein which is structurally and functionally similar to avidin but with improved properties, such as better affinity towards biotin conjugate, useful immunological properties or faster biotin dissociation rate, compared to avidin and streptavidin....
Avidin-like proteins from symbiotic bacteriaAn isolated protein which is structurally and functionally similar to avidin but with improved properties, such as better affinity towards biotin conjugate, useful immunological properties or faster biotin dissociation rate, compared to avidin and streptavidin....
Developing a monomeric form of an avidin‐like protein with highly stable biotin binding properties has been a major challenge in biotin‐avidin linking technology. Here we report a monomeric avidin‐like protein—enhanced monoavidin—with off‐rates almost comparable to those of multimeric avidin ...
Hunt, LT, Barker, WC (1989) Avidin-like domain in an epidermal growth factor homolog from a sea urchin. FASEB Jour 3: pp. 1760-1764Hunt, L. T. and Barker, W. C. (1989) Avidin-like domain in an epidermal growth factor homolog from a sea urchin. FASEB J. 3, 1760-1764...
Embodiments described herein relate to methods for reversible binding of a biotin-binding protein, e.g., rhizavidin, including fusion proteins and complexes thereof to a matrix comprising a lipoic acid (LA) compound or derivative thereof immobilized to a solid support, where the biotin-binding ...
Monomeric avidin-like proteins capable of stable binding to biotinThe present invention is a kind of dimension Oudin more inequal than class D quasiprotein and two kinds with powerful and stable dimension Oudin binding function, the method that nucleic acid with protein consultant, clear breast type...
Embodiments described herein relate to methods for reversible binding of a biotin-binding protein, e.g., rhizavidin, including fusion proteins and complexes thereof to a matrix comprising a lipoic acid (LA) compound or derivative thereof immobilized to a solid support, where the biotin-binding ...