DNA-Binding ProteinsRecombinant Fusion ProteinsTwo-Hybrid System TechniquesProtein Structure, TertiaryThe transcriptional response to auxin is critical for root and vascular development during Arabidopsis embryogenesis. Auxin induces the degradation of AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional ...
The algal ABP1-like protein and their land-plant counterparts share the conserved motif that forms the auxin-binding pocket (Figure 2C). Show abstract Why plants need more than one type of auxin 2011, Plant Science Citation Excerpt : Exactly how these two mechanisms cooperate remains to be ...
Abel S, Theologis A (1995) A polymorphic bipartite motif signals nuclear targeting of early auxin-inducible proteins related to PS-IAA4 from pea (Pisum sativum). Plant J 8:87–96 PubMed CAS Google Scholar Ahmad S, Gordon-Weeks R, Pickett J, Ton J (2010) Natural variation in priming...
Regions flanking the canonical GWPPVR degron motif influence AUX/IAA protein recruitment by the SCFTIR1, impact auxin binding, and AUX/IAA degradation24,34,35,36,37. A broader sequence context of the AUX/IAA degron might be crucial for the adequate regulation of AUX/IAA processing and turnove...
An SPR study (Quareshy, Uzunova, Prusinska, & Napier, 2017) employed the auxin receptor, TIR1, and related F-box proteins (F-box is a ~ 50 amino acid structural motif that mediates protein–protein interactions) to screen for the design of novel compounds with auxin activity. Advanced ...
semi-technical replicates in independent biological replicates (independently transformed lines,n = 4).dDual luciferase transactivation assay in Arabidopsis protoplasts using aLUCgene under the control of 5xGal UAS motif as reporter,35S:RENas ratiometric control, and the yeast Gal4 DNA binding doma...
Genome-wide binding site analysis of maize ARFs Full size image All ARFs preferentially bound to sequences containing the core TGTC motif, however clade A ARFs predominantly showed enrichment for the motif TGTCGG as observed previously for Arabidopsis12,17, whereas most clade B ARFs showed enrichmen...
Loci regulated by ZF1 or ZF10 were enriched for a CTCF binding motif associated with its respective binding We hypothesized that genes whose regulation by CTCF was dependent on ZF1 or ZF10 for chromatin binding might require additional DNA signatures unique to each ZF, in addition to the core...
(Supplementary Fig.3d). ChIP qPCR revealed that FIL bound to theSTMlocus, with the strongest binding observed at the proximalSTMpromoter (Fig.5d), a conserved region with a FIL binding motif41. FIL also bound to theBPclass I KNOX gene locus (Supplementary Fig.5i). These data suggest that...
1995. A polymorphic bipartite motif signals nuclear targeting of early auxin-inducible proteins related to Ps-IAA4 from pea (Pisum sativum). Plant J. 8: 87–96. PubMed CAS Google Scholar Abel, S. and Theologis, A. 1996. Early gene and auxin action. Plant Physiol. 111:9–17. Pub...