IntelliQube brochure IntelliQube manual Scale your PCR diagnostic testing to high-throughput application note IntelliQube real-time quantitative PCR performance characterisation KASP™ Array Tape Master mix manual Related products KASP Array Tape Master mix...
Finally, to limit the human intervention in the automated workflow for plasmid construction, it is important to confirm the correct PCR amplification, the number of colonies being generated, and the digestion pattern at the end of each module, respectively (Supplementary Fig.14and Supplementary Table...
Using a reservoir and multichannel pipettor, 54 µl of 0.15% SDS neutralization solution was added to each well, followed by 4 µl of premixed i5 and i7 barcoded primers and 36 µl of premixed KAPA PCR Master Mix. The plate was then sealed and returned to a thermocycler for...
3. Add 2,400 µL of the QIAGEN 2X PCR Master Mix to the 15-mL tube. • All three QIAGEN 2X Multiplex PCR Master Mix supplied vials will be needed. • It is recommended to set a P1000 single channel pipette to 800 µL and remove this volume from the first vial. • ...
Said detection antibodyDirectly or indirectly attached to signal DNAMultiple chambersIncludes a chamber containing PCR Master MixMultiple chambersA chamber comprising primers for amplifying all or one regions of said signal DNAMultiple chambersA chamber comprising probes for detecting all or one regions ...
Template genomic DNA, primers, and hydrolysis probes were added to a PCR master mix (SsoFast Universal Probes mix (BioRad, Mississauga, ON, Canada) to final concentrations of 176 nM for probes and 500 nM for each primer. Real-time PCR assays were performed with a temperature profile of ...
Thermo Fisher Scientific 的微生物组试剂盒提供了一种专用解决方案,可快速轻松地从粪便和土壤中纯化高质量核酸。这些试剂盒采用了MagMAX 磁珠技术,确保可重复地回收高质量核酸,适用于微阵列、实时 PCR 和下一代测序等广泛应用。 无论样品类型如...
(PCR plate type must be specified on setup form under step 2) Empty PCR plate seated on red insert (PCR plate type must be specified on setup form under step 2) Total RNA samples in twin.tec plate Master Mix Source Plate seated on silver insert (Nunc DeepWell; see Figure 3 on page ...
Duplex PCR reactions were run in parallel to detect P. falciparum and P. vivax, the 20 μl reaction mix contained 2 μl DNA, 1× TaqMan Master Mix (Applied Biosystems, USA), 200 nM forward and reverse primer, 200 nM P. falciparum probe and 100 nM P. vivax probe. The PCR programme...
Experiments and data analysis were performed on a Taqman ABI 7900 real-time PCR machine using the Power SYBR Green PCR Master Mix (Life Technologies) following the manufacturer's instructions. The ALG9 gene was chosen as the reference gene (one copy on the chromosome), and the integration ...