Methods for refining the compositions of bacterial growth media to improve heterologous expression of desired recombinant target genes are provided. Also provided are compositions and culture media obtained using the above methods.FOX BRIAN G.BLOMMEL PAUL G....
· H O, μMNiCl ·6H O, μMNa MoO ·5H O, μMNa SeO ·5 H O,and μMH BO . Vitaminmixtureusedwiththeauto- inductionmedia(toachieve inal concentrations): . μMnicotinicacid, . μMpryridoxine, . μMthiamine, . μMp-aminobenzoicacid, . 5 μM olicacid, . 5μMribo avin, ....
产品编号: N990-1L 查看说明书 产品名称: ZYP-5052, Autoinduction Media .0 订购此产品 供应商: Amresco 规格: 目录价: 992 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址: 点击查看详细 打印此页 关闭此页 上一个:现货|BCS ASSAY KIT[N962-SAMPLE/] 下一个:...
We report a simple and cost-effective autoinducible media component responsible for the autoinduction of proteins in Escherichia coli under lacUV5 promoter system. Yeast extract (YE) at high concentration was found to stimulate the expression of T7 RNA polymerase in BL21(DE3) cells while such an...
coverage per mg coral tissue (ANOVA, p = 0.0232) (Fig. 2b) (Supplementary Table S2). Regardless of CFCF, diseased plated homogenates produced significantly more bacterial growth than did healthy plated homogenates on TCBS media. Healthy plated homogenates produced no culturableVibriospp. (Fig...
Auto-inducing media have been developed for labeling proteins with selenomethionine, 15N or 13C, and for production of target proteins by arabinose induction of T7 RNA polymerase from the pBAD promoter in BL21-AI. Selenomethionine labeling was equally efficient in the commonly used methionine auxo...
These unique culture media are based on technology by F. William Studier at Brookhaven National Laboratory. The products include Overnight Express Instant TB Medium, System 1, and System 2. The granulated Instant TB Medium formulation combines Overnight Express System 1 and Terrific Broth (TB) ...
The Fosmid Autoinduction protocol improves upon the existing induction protocol by including the autoinduction supplement in the media prior to culture inoculation, removing the need for time-consuming subculturing and the 2-hour incubation required in the standard induction protocol. The Fosmid Autoindu...
induction media, demonstrating its high potential as a facile method for production of recombinant SELPs in small/laboratory scale fermentations, but also for the production of several other proteins. In fact, in our laboratory, several other recombinant proteins were produced by this auto-induction...
ZYP-5052自诱导培养基/ZYP-5052, Autoinduction Media 商品编号: N990-1L 品牌: Amresco 规格: N990-1L CAS: # 库存: 20 售价: ￥541.00 购买数量 -+ 产品介绍Product Description 规格:N990-1L 分类:生化检测 储存条件:RT 用途:分子生物学实验 注意事项:...