Membranes were blocked with 5% skimmed milk/TBS pH 7.6/0.1% Tween20 or 5% skimmed milk/0.1 M NaCl/1 M Tris pH7.4/0.1%Tween 20 for 1 h at room temperature and incubated with antibodies against Survivin (Abcam, Cambridge, UK), Aurora B (Abcam), INCENP (Cell Signaling Technology), p...