Selectivity of intracellular proteolysis: protein substrates activate the ATP-dependent protease (La) Science. 1986; 232 :500–503.Waxman,L. and Goldberg,A.L. (1986) Selectivity of intracellular proteolysis: protein substrates activate the ATP-dependent protease (La). Science, 232, 500-503....
英文名称:Listeria monocytogenes serotype 4b ATP dependent protease subunitListeria monocytogenes serotype 4b ATP dependent protease subunit is available 12 times 来自 Mbs recombinant la,中文名称:单核细胞增生李斯特菌4b型ATP依赖性
Genome sequencing of six resistant mutants (Figure 4) showed mutations in the clpC1 gene, which encodes the subunit of the hexameric ATPase complex, ClpC1. It functions in protein degradation with the two-ring protease complex, ClpP1P2, in mycobacteria (Akopian et al., 2012, Raju et al.,...
L-methionine also eliminated the difference in the growth rates between the protease- deficient control WE(P-) and mutant Y229(P-) strains (0.58 and 0.59 h-1, respectively) at 42°C (Additional file 5: Table S3; Additional file 7: Figure S5). However, the protease-negative mutants grew...
This has been attributed to the loss of mtDNA5 integrity in Pim1-deficient cells, resulting in a ρ− phenotype (6, 7). The molecular mechanisms responsible for the loss of mtDNA integrity in Pim1 mutants are still elusive, but the proteolytic function of Pim1 protease has been implicated...
L. 1982. Protease La from E. coli hydrolyzes ATP and proteins in a linked fashion. Proc. Nat. Acad. Sci. U.S.A. 79:4883–4887. Google Scholar Desautels, M., and Goldberg, A. L. 1982. Liver mitochondria contain an ATP-dependent vanadate-sensitive pathway for degradation of proteins....
To monitor protein levels, frozen muscles were dissolved by means of Qiagen Tissue Lyser and protein extracts were prepared in a buffer containing: 50 mM Tris pH 7.50, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 10% glycerol, 2% SDS, 1% Triton X-100, Roche Complete Protease Inhibitor...
BMDNs and splenocytes from WT and Panx1−/− mice were incubated in RIPA lysis buffer (1 mM PMSF, 0.2% Na-deoxycholate, 2 mM Na-orthovanadate, 10 mM NaF, 1X Roche Protease Inhibitor, 0.05% SDS, 1X NP40, 1 mM EDTA, pH 7.4.) for 15 minutes on ice. Samples were...
The results indicate that FtsH has an ATP-dependent protease activity with high substrate specificity. MATERIALS AND METHODS Bacterial Strains and Media CU141 (MC4100 carrying F′lacIq) (19, 20) and its ompT::kan derivative, TYE024 (9), were described previously. AD367 (MC4100, zhj-6198:...
Proteins made in such systems may be designed to include heparin, thrombin, or factor XA protease cleavage sites so that the cloned polypeptide of interest can be released from the GST moiety at will. In the yeast, Saccharomyces cerevisiae, a number of vectors containing constitutive or ...