The cleaved protein was passed through Ni-NTA resin to remove the 6xHis-GST fusion protein and TEV protease prior to size exclusion chromatography on a prep-grade Superdex S200 (Cytiva) column in 50 mM Tris pH 8.0, 500 mM NaCl, 1 mM TCEP, and 5% glycerol. Fractions containing ...
For DNA digestion of single and double-stranded DNA, 1 μg RNA sample was incubated at room temperature for 10 min with 1 μl 10× DNase I Reaction Buffer, 1 μl DNase I Amp Grade 1 U/μl, and DEPC-treated water to final volume of 10 μl. DNase I was inactivated by incubation ...
ATP synthesis and thermogenesis are two critical outputs of mitochondrial respiration. How these outputs are regulated to balance the cellular requirement for energy and heat is largely unknown. Here we show that major facilitator superfamily domain cont
Southern California-based prospective cohort data were collected from 1164 individuals who had used nicotine products at some point in their lives, with surveys administered during their 12th grade year (T12016), and 24 months (T2) and 42 months (T3) later. In each survey, the past 30 days...