Bound nucleotide was removed via apyrase digestion (New England Biolabs) in the presence of 2 mM dithiothreitol (DTT) and 4 mM CaCl2, overnight at room temperature, followed by another run on a Superdex 200 column. Protein concentrations were determined photometrically. GST-tagged full-...
Hg2+,MeHg Biotissues Comparison of two spray chamber-nebulizer systems 75 cm × 75 μm, 25 kV 25 mM CaCl2 ICP-MS 112 and 149 ng ml−1 with cross-flow nebulizer; 6.0 and 13.6 ng ml−1 with MCN-100 nebulizer 178 Hg2+,MeHg,EtHg Contact lens solution Microconcentric nebulizer as int...