at 4 °C, pellets were resuspended in 1 ml cold 'lysis buffer' (50 mM Tris. pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, 1% Triton X-100 and 1 tablet mini complete) and incubated for 20 min on ice. After 5-min centrifugation at 1,800 r.p.m. at 4 °C, pellets were ...
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A simple and cost effective RP-HPLC method is developed for simultaneous estimation of glimepiride (GLIM) and metformin (MET) at tablet dosage form using C-18 column (4.6 x 250mm, 5μ, 100 A˚) with a mobile phase composed of methan... SDS Kumar,P Nakasthra,K Mamatha,... 被引量...
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