Bradford Institute for Health Research, Bradford, UK Dan Mason & John Wright Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia Allan F. McRae, Tian Lin, Grant W. Montgomery, Naomi R. Wray & Peter M. Visscher ...
(NE-PER kit, Thermo Scientific) and homogenized using a tissue grinder. Nuclear proteins were extracted in accordance with the manufacturer’s instructions. Protein concentrations were determined using the Bradford method (Bio-Rad SmartSpec Plus, Bio-Rad Laboratories, Inc., USA). Approximately 0.15...
Supernatant was transferred to a new tube; protein concentration was measured by Bradford assay. Antibody-conjugated beads were washed 3 times in blocking buffer, resuspended in 50μL blocking buffer and added to 500μg input protein for overnight incubation with rotation at 4°C. Fifty ...
(DTE) pH = 7.6]. A Bradford assay was performed to determine the protein concentration. According to the aforementioned assay, 200–500 μg of the sample were transferred to an Amicon Ultra 0.5 Centrifugal Filter Device (Merck, Darmstadt, Germany) and samples were centrifuged for 15 min at ...
Li B, Verma SS, Veturi YC, Verma A, Bradford Y, Haas DW, Ritchie MD. Evaluation of PrediXcan for prioritizing GWAS associations and predicting gene expression. In: Pacific Symposium on Biocomputing 2018: Proceedings of the Pacific Symposium. 2018. p. 448–59. Hirbo J, Bailey JC, Evans ...