Sequencing libraries were prepared with NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions. Libraries were then sequenced on the Illumina HiSeq 4000 system using the 150-bp paired-end sequencing protocol. Quality assessment of raw data with ...
After all timepoints were collected, DNA, RNA, and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform. Proteins were quantified with the Pierce™ Rapid Gold BCA Protein Assay Kit...
But all the prep work involved makes it pay less than my day job. Maybe I can improve on that next course. We'll see. I haven't decided my next target for hacking yet. I'll have to decide that when I get back from vacation. Ciao, @ [] permanent link / / December 2024 ...
The hidden butler's pantry houses the microwave, ice maker and prep sink along with a Costco door and tons of extra storage. Upstairs, the luxurious owner's suite is a haven with a spacious wet room, soaker tub, double vanities, extra large walk-in closet and private deck access. In ...
COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas Cell, Volume 184, Issue 23, 11 November 2021, Pages 5838 Xianwen Ren, Wen Wen, Xiaoying Fan, Wenhong Hou, Bin Su, Pengfei Cai, Jiesheng Li, Yang Liu, Fei Tang, Fan Zhang, Yu Yang, Jiangping He, Wenj...
CD4+ T cells are key regulators of the adaptive immune system and can be divided into T helper (Th) cells and regulatory T (Treg) cells. During an immune response Th cells mature from a naive state into one of several effector subtypes that exhibit disti
RNA libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (NEB) with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) to enrich for poly-A-tailed RNA molecules. RNA-seq library quality was analyzed by BioAnalyzer (Agilent Genomics) and quantified using qPCR (Kapa ...
(middle), and bar plots show the number of cells/nuclei profiled for each tissue after quality control (right).n = 1,144,706 individual cells/nuclei analysed.b, UMAP visualization of all clusters coloured by major cell types. A total of 113 cell clusters were identified in the ...
Plasticity in gene expression allows bacteria to adapt to diverse environments. This is particularly relevant in the dynamic niche of the human intestinal tract; however, transcriptional networks remain largely unknown for gut-resident bacteria. Here we
Nuclei isolated using the EZ-Prep kit from entorhinal cortex of AD and control patients were FACS sorted on BD Influx using 70 μm nozzle, 21–22 psi. Single DAPI+ events were considered nuclei. Extended data Fig. 2 Single nuclei metadata and analysis of hybrid cells. a, cell proportion ...