After 24 h, cells were transfected with 50 or 100 nM of p75NTRsiRNA (Dharmacon Inc., Lafayette, CO, USA) or scrambled RNAi as mock control, combined with Lipofectamin 2000 and Opti-MEM (both from Invitrogen Corporation) as datasheet suggests. Cells were transfected twice and used for FACS...
After 24 h, cells were transfected with 50 or 100 nM of p75NTRsiRNA (Dharmacon Inc., Lafayette, CO, USA) or scrambled RNAi as mock control, combined with Lipofectamin 2000 and Opti-MEM (both from Invitrogen Corporation) as datasheet suggests. Cells were transfected twice and used for FACS...