用ATHB12基因的引物ATHB12-F和ATHB12-R扩增得到长度约900 bp的PCR扩增片段(图1), 将PCR产物切胶回收后连接至载体pMD18-T, 转化大肠杆菌DH5α , 提取质粒测序表明其序列与马铃薯ATHB- 12-like(GenBank登录号为XM_006339695)同源性达到100%。
ATHB12 ,like ATHB7 ,was up-regulated during water deficit conditions, the up-regulation being dependent on abscisic acid (ABA) and on the activity of the Ser/Thr phosphatases ABI1 and ABI2. Plants that are mutant for ATHB12 , as a result of T-DNA insertions in the ATHB12 gene, ...
In another report, loss-of-function athb7 and athb12 mutants have revealed that both genes activate clade A protein phosphatases 2C (PP2C) genes and reppress PYL5 and PYL8 (Pyrabactin Resistance 1-like 5 and 8), ABI1 (ABA Insensitive 1), ABI2 (ABA Insensitive 2), HAB1 (...
ATHB17 was reported as a transcriptional repressor containing EAR (ERF-associated amphiphilic repression)-like motif35. Consistent with this result, we found that ATHB17 repressed the transcription of 26 PhANGs under normal or salt stress conditions (Table 2 and Fig. 4), with 5 of them (...
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BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3 Nature, 425 (2003), pp. 316-321 Google Scholar Yamaguchi-Shinozaki and Shinozaki, 1993 K. Yamaguchi-Shinozaki, K. Shinozaki Characterization of the expression of a desiccation-responsive rd29 gene...
ATHB12 expression is correlated with an array of morphological abnormalities including leaf curling, stunting, and callus-like structures in infected Arabidopsis. Inoculation of plants with a BSCTV mutant with a defective c4 gene failed to induce ATHB12. Transgenic plants expressing the BSCTV C4 ...
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In another report, loss-of-function athb7 and athb12 mutants have revealed that both genes activate clade A protein phosphatases 2C (PP2C) genes and reppress PYL5 and PYL8 (Pyrabactin Resistance 1-like 5 and 8), ABI1 (ABA Insensitive 1), ABI2 (ABA Insensitive 2), HAB1 (...