Scott Landers founded ATG (Applied Technology Group) to bridge the gap between functional hardware and cutting-edge software in the AEC industry – first by providing computers tailored to industry workflows, and secondly with software needed to get the work done as we entered the digital age. ...
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Intracellular recycling via autophagy is governed by post-translational modifications of the autophagy-related (ATG) proteins. One notable example is ATG4-dependent delipidation of ATG8, a process that plays critical but distinct roles in autophagosome f
Fig. 3: STX18 negatively regulates the interactions of ATG14-ATG8s and the formation of PI3KC3-C1 complex. aPI(3)P production was analyzed by the ELISA assay. The fold change was calculated based on the concentration of PI(3)P and normalized against control group.bThe effect of serum st...
Large unilamellar vesicles with a diameter of around ~140 nm were prepared by extrusion of the rehydrated phospholipid suspensions with 21 strokes through a 0.1 µm polycarbonate membrane (Millipore Inc., Bedford, MA, USA) with a mini-extruder (Avanti Polar Lipids). Homogeneity of ...
Large unilamellar vesicles with a diameter of around ~140 nm were prepared by extrusion of the rehydrated phospholipid suspensions with 21 strokes through a 0.1 µm polycarbonate membrane (Millipore Inc., Bedford, MA, USA) with a mini-extruder (Avanti Polar Lipids). Homogeneity of ...
The level of ROS in OVX MMSCs-M was elevated compared with sham group (Fig. 6a). Moreover, to further explore the role of endogenous ROS in miR-152-5p/ATG14-mediated autophagy promoting osteogenic differentiation, H2O2 (100um) was applied to OVX MMSCs-M in order to increase the ROS ...
Based on these initial results, we then increased the amount of tissue lysate applied to the DNA isolation col- umn to the maximum recommended, omitted the 90 °C FFPE step, obtained cadaver skeletal muscle and skin samples as other tissue sources of DNA, and, in order to rule out the ...
and the Guangdong Basic and Applied Basic Research Foundation (2022A1515010856 to Y.W.). L.Z. was also supported in part by a Presidential Fellowship. Y.W. and R.T. were supported by a Ganghong Young Scholar Development Fund at the Chinese University of Hong Kong, Shenzhen. The ...
Auto- mated image analysis algorithms were applied in Matlab (r2019a, Mathworks, Natick, MA) to determine tumor nodule area and total tissue area. Immunohistochemical staining of ATG16L1 ATG16L1 protein was detected by immunohistochemical staining of formalin-fixed paraffin-embedded tissue per ...