Twenty fields were acquired per condition, a single focal plane by field. The ImageJ plugin, co-localization colour map was used for automatic quantification and visualization of co-localized fluorescent signals. Brightness and contrast was adjusted for all images to exclude background signal. Before...
bright-field images showing the morphology of WT and ATF6-deficient hMSCs differentiated from WT and ATF6-deficient hESCs, respectively. Scale bar, 50 μm.fWestern blotting showing the protein level of ATF6 in WT and ATF6-deficient hMSCs. β-Actin was used as the loading control. See al...