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To generate a parasite strain with a mutation at the 352 position, we used the pl0001 plasmid to generate the allelic replacement construct of PbTatD-D352A. The fragment (–1,500 to –1,000 bp) was amplified and inserted at the 5′ end of the DHFR-TS cassette. The fragment (–1...
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The fragment (–1,500 to –1,000 bp) was amplified and inserted at the 50 end of the DHFR-TS cassette. The fragment (–1,000 to þ 1,389 bp with mutation) was chemically synthesized by Sangon Biotech (Shanghai Co., Ltd) and was inserted at the 30 end of the DHFR-TS cassette...