Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC30-2200)Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)Trypsin Neutralizing Solution (ATCCPCS-999-004)Gentamicin-Amphotericin B Solution (ATCCPCS-999-025)Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)Phenol Red (ATCCPCS-999-...
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 ) Trypsin Neutralizing Solution (ATCC PCS-999-004 ) Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 ) Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 ) Phenol Red (ATCC PCS-999-001 ) ...
在解离前将用于原代细胞的胰蛋白酶-EDTA(ATCC PCS-999-003)和胰蛋白酶中和溶液(ATCC PCS-999-004)温热至室温。在与细胞一起使用之前将*生长培养基加热至37℃。 对于每个烧瓶,小心地吸出用过的培养基而不打扰单层。 用3至5mL D-PBS(ATCC 30-2200)冲洗细胞层一次以除去残留的培养基。 向每个烧瓶中加入预热的...
Add trypsin for primary cells (ATCC PCS-999-003), 1 mL/25 cm2. Place at 37oC for 3-4 minutes, until 90% of the cells have detached. Rapflask gently to ensure cells are detached. Add 2% FBS in D-PBS, 1 mL/25 cm2to neutralize trypsin. ...
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC30-2200)Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)Trypsin Neutralizing Solution (ATCCPCS-999-004)Gentamicin-Amphotericin B Solution (ATCCPCS-999-025)Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)Phenol Red (ATCCPCS-999-...
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 ) Trypsin Neutralizing Solution (ATCC PCS-999-004 ) Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 ) Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 ) Phenol Red (ATCC PCS-999-001 ) ...