yinxx/V2ray-ConfigsPublic forked frombarry-far/V2ray-Configs NotificationsYou must be signed in to change notification settings Fork0 Star0 Files .github Base64 Files Licence Splitted-By-Protocol All_Configs_Sub.txt All_Configs_base64_Sub.txt ...
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(final concentrations in 15 ul volume): 0.2 µM of each primer 1 U/µl Taq polymerase (Fermentas) 0.1 mM dNTPs 2.5 mM MgCl2 1 × (NH4)2SO4 PCR buffer 4 µl of 10x diluted DNA H-2Eb MusDRBF2 JS1 PCR cycling conditions: 94°C (2 min) initial denaturation 32 cycles of: ...
Polymerase chain reaction amplification was carried out in 20 ml reaction volume containing 2 ml of 10 Â ZenonBio, 10 Â reaction buffer, 50 nM of each of the primers, 0.5 mM of each of the dNTPs, 4 mM MgCl2, 100 ng of DNA extract and 0.3 U of ZenonBio TaqPoly- merase. The...
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Defining chromosomal deletion breakpoints GoTaq Long (Promega, Southampton, UK) enzyme was utilized for all long- range PCR assays. Using co-ordinates derived from SNP array genotyping data, the following primers were designed to span the predicted deletion breakpoint in proband C27; ZEB1_C27_F...
All qPCRs were performed on a RotorGene 6,000 using MyTaq HS DNA polymerase with the addition of 5 mM of Syto9, annealing temperature of 66 °C and extension of 30 s. Each experiments was performed three times in duplicate. The BAC clone (CTD-2278I10) covering the 19p13 region was ...
religions Article Glocal Religion and Feeling at Home: Ethnography of Artistry in Finnish Orthodox Liturgy Tatiana Tiaynen-Qadir Faculty of Social Sciences, University of Turku, Turku 20014, Finland; tatiana.tiaynen@ttaq.fi Academic Editors: Victor Roudometof and Peter Iver Kaufman Received: 19 ...
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