We used HALLMARK, KEGG and REACTOME gene sets from the Molecular Signature Database (MsigDB: http://www.broad.mit.edu/gsea), kept pathways with a q-value <0.1 and absolute average log2 fold changes >0.5, and ignored those with more than 10% ribosomal genes. For the diffusion map, we ...
Figure 2 KEGG pathway analysis of differentially expressed mRNAs in ovaries of Hu sheep at (A) H1 vs H3 and (B) H3 vs H8. H1, H3 and H8 represent ovaries sampled at 1, 3 and 8 months old, respectively. The x-axis represents the name of the pathway, and the y-axis represents the...
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The tools for ligand-receptor interaction at single cell level are collated in Table2. These analytical tools and methods greatly facilitate researchers in quantifying the statistical significance of cell–cell interactions and reveal the potentially critical ligand-receptor pairs mediating such interactions....
The KEGG pathway enrichment analysis was also subjected to a Fisher's exact test with FDR < 0.05. 2.4. Real time–quantitative PCR (RT-qPCR) analysis Total RNA (1 μg) extracted from pericarp of the genotypes M82, yft1, YFT1-CDS, and YFT1-RNAi tomatoes, at 35 dpa, 47 dpa and 54...
为了系统地评估物种间的总体趋势,使用GO和KEGG分析来描述这五个物种中随年龄变化的基因,并整合功能富集结果(图3D和E)。GO和KEGG富集结果表明,这些基因参与了相同的途径,可能在物种间具有相似的功能。例如,三种脊椎动物中最常见的上调基因与免疫反应、应激反应调节等相关(图3D)。另一方面,五个物种中随年龄降低而下调...
Pathway-Express (PE) is a pathway analysis tool in the Onto-Tools ensemble utilizing the available Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway information [17, 19]. This tool generates, firstly, a probability (p) value based on the number of genes present in a particular pathway....
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. Of particular note, 5 of these transcripts were up-regulated in SMAv motor neurons compared to SMAr (Fig.9a). KEGG pathway analysis revealed that a number of these up-regulated transcripts pertain to the P53 signalling pathway. Specifically, the RNAseq results indicated a 4.8 Log2fold and ...
data of flow cytometry analysis revealed the effect of MGCG knockdown or overexpression on apoptosis of LN229 cells.HThe data of TUNEL analysis demonstrate the effect of MGCG knockdown or overexpression on apoptosis of LN229 cells.ITop 10 KEGG pathway enrichment of MGCG.JWestern blot analysis ...