We used HALLMARK, KEGG and REACTOME gene sets from the Molecular Signature Database (MsigDB: http://www.broad.mit.edu/gsea), kept pathways with a q-value <0.1 and absolute average log2 fold changes >0.5, and ignored those with more than 10% ribosomal genes. For the diffusion map, we ...
ACE2: Angiotensin I converting enzyme 2 PT: Proximal tubule cells Tfh: Follicular helper T cells scTCR-seq: Single cell T cell receptor sequencing TCR: T cell receptor CSF: Cerebrospinal fluid GOCC: Gene Ontology Cellular Compartment KEGG: Kyoto Encyclopedia of Genes and Genomes TSK...
Figure 2 KEGG pathway analysis of differentially expressed mRNAs in ovaries of Hu sheep at (A) H1 vs H3 and (B) H3 vs H8. H1, H3 and H8 represent ovaries sampled at 1, 3 and 8 months old, respectively. The x-axis represents the name of the pathway, and the y-axis represents the...
Construction of a cross-species cell landscape at single-cell level 细胞是生命的基本单位,尽管有研究表征不同生物的细胞异质性,但是全面的动态的跨物种比较尚未实现。 此研究使用scRNA-seq绘制小鼠、斑马鱼和果蝇多个生命阶段的细胞图谱。通过整合 > 260万个单细胞的数据集,构建了跨物种的细胞图谱,并确定了在整个...
The FDR < 0.05 and |log2 (FC) |≥ 1 were set as the threshold for the identification of significant differential expression. The differentially expressed genes (DEGs) were subjected to enrichment analysis of GO and KEGG pathways. RT-qPCR validation RT-qPCR was performed to validate the...
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These transcripts were annotated to NT, NR, GO, COG, KEGG, SwissProt, and InterPro databases using Blastn, Blastx, Diamond, Blast2GO, and InterProScan5 software. The RSEM package was used to measure gene expression level. Differential expression analysis was performed using DEseq2 method. ...
Marine bacteria Vibrio diabolicus strain L2-2 has been proven to be able to transform SMX. In this study, the potential resistance and biotransformation mechanism of strain L2-2 to SMX, and key genes responses to SMX at environmental concentrations were researched. KEGG pathways were enriched by ...
9a). KEGG pathway analysis revealed that a number of these up-regulated transcripts pertain to the P53 signalling pathway. Specifically, the RNAseq results indicated a 4.8 Log2 fold and 5.9 Log2 fold up-regulation of PMAIP and CDKN1a respectively. This up-regulation was confirmed by qPCR on ...
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