Genetic deletion of ERK1 results in a viable mouse with normal brain histology but subtle-behavior alterations. Because deletion of ERK2 is embryonic lethal we have taken a conditional knockout approach to selectively delete ERK2 in the CNS by crossing nestin-Cre mice with those harboring a ...
All surgical pro- cedures were carried out in the Department of Anatomy and Histology/Embryology, KMU. The surgical proce- dures for middle cerebral artery occlusion (MCAO) fol- lowed that described previously by us [43, 44]. Briefly, the animals were anaesthetized with an intraperitoneal injec...
The mechanistic functions of FTY720 were investigated in聽vitro and in聽vivo utilizing immunofluorescence, histology, MRI and behavioral analysis. The in聽vitro study showed that FTY720 can reduce astrocyte migration and proliferation activated by S1P. FTY720 can prolong internalization of S1P1 and exert...
Vascular damage and astrocyte and microglia activation were examined using histology and immunohistochemistry. Quantification was performed using HALO image analysis software. Compared to injured normotensive mice, injured hypertensive mice exhibited significantly more IgG extravasation in the cortex denoting a ...
Specimens of neocortical samples were processed routinely for paraffin embedding and sections of 6 渭m thickness stained for neurohistology. Another set of specimens was cryosectioned at 23 掳C after cryoprotection in 30% sucrose/PBS and evaluated for GFAP immunohistochemistry. The study showed a ...