Purified neutrophils (6 × 105) in 200 μl conditioned medium (CM) were seeded into the upper chambers of transwell plate containing a polycarbonate filter (3 μm pores, Corning, UK). CM (500 μl) was added into the lower chambers in a 24-well trans-well plate. To detect neutroph...
We performed a meta-analysis combining asthma GWAS results from Iceland (n = 16,247 cases,n = 346,486 controls) and the UK biobank (n = 52,942 cases,n = 355,713 controls). The asthma phenotype in both sets was based on physician diagnoses and/or self-reported doctor...
B Distribution of common phyla and genera in the airways of healthy and asthmatic subjects: The graph depicts the relative abundance (in %) of the five most common phyla of bacteria colonizing the human airways and lung in healthy (white bars) and in asthmatic (black bars) subjects. Phyla...
Gene symbols are indicated at the top of each graph. The top horizontal bar illustrates the location of SNPs on a physical scale. The color of squares illustrates the strength of pairwise r2 values on a black and white scale where black indicates perfect LD (r2 = 1.00) and white indicates...
PE-Cy5 conjugated F4/80 (Cl:A3-1(F4/80)) was obtained from Serotec Ltd., Oxford, UK. CFU-c assay To quantitate committed progenitors of all lineages, CFU-C assays were performed using methylcellulose semisolid media (Stemgenix, Amherst, N.Y.) supplemented with an additional 50 ng of ...
Globally, however, this may not as effective as hoped, as despite prioritising symptom and peak flow diaries as core components of asthma apps, in reality few patients engage regularly with monitoring tasks60. Future studies will need to explore the feasibility of using digital support for self-...
1 hr before the challenge: POM1 (20 mg/kg; Tocris Bioscience, Bristol, UK), anti-CD9 antibody (5 mg/kg; 553758, KMC8, BD Pharmingen, San Diego, CA, USA), anti-IL-17/IL-17A antibody (250 µg/kg; 50104, R&D Systems, Minneapolis, MN, USA), and GSK484 (PAD4 inhibitor; 4 ...
Briefly, the trachea was cannulated and bronchoalveolar lavage fluid (BALF) was obtained via lavage with 0.3 ml RPMI 1640 (Invitrogen, UK) 3times. The lavage fluid was pooled. BALF total white blood cell counts were performed using an automated cell counter (Sysmex F-820, Sysmex UK Ltd). ...
FIG. 9 is a graph showing the in vivo proliferation of SW1990 mock cell line injected into a nude mouse which had previously been injected with SW1990 si cell line. FIG. 10 is a graph showing the production of PGE2 by a stimulus of SW1990 mock cell line in which MUC5AC is expressed...
UK patent application GB 2 258 234 A, published Feb. 3, 1992, teaches a soluble Kit ligand (KL) protein. European patent application 0 423 980 A1, published Apr. 24, 1991, teaches stem cell factor and methods of use for treating blood disorders. ...