Purified neutrophils (6 × 105) in 200 μl conditioned medium (CM) were seeded into the upper chambers of transwell plate containing a polycarbonate filter (3 μm pores, Corning, UK). CM (500 μl) was added into the lower chambers in a 24-well trans-well plate. To detect neutroph...
Notably, China (169, 21.8%), the USA (137, 17.6%), Japan (125, 16.1%), the UK (99, 12.7%), and South Korea (46, 5.9%) are more prominent than others. The contacts and cooperation between countries were depicted in Figures 3 and 4 using CiteSpace and VOSvi...
We performed a meta-analysis combining asthma GWAS results from Iceland (n = 16,247 cases,n = 346,486 controls) and the UK biobank (n = 52,942 cases,n = 355,713 controls). The asthma phenotype in both sets was based on physician diagnoses and/or self-reported doctor...
PE-Cy5 conjugated F4/80 (Cl:A3-1(F4/80)) was obtained from Serotec Ltd., Oxford, UK. CFU-c assay To quantitate committed progenitors of all lineages, CFU-C assays were performed using methylcellulose semisolid media (Stemgenix, Amherst, N.Y.) supplemented with an additional 50 ng of ...
Gene symbols are indicated at the top of each graph. The top horizontal bar illustrates the location of SNPs on a physical scale. The color of squares illustrates the strength of pairwise r2 values on a black and white scale where black indicates perfect LD (r2 = 1.00) and white indicates...
Finally, Huang et al. showed thatLactobacillus paracasei(LP),Lactobacillus fermentum(LF), or their combination, given to asthmatic children aged 6 to18 years as capsules for 3 months, lowered the asthmatic severity, improved asthma control, increased the peak expiratory flow rate, and decreased ...
Globally, however, this may not as effective as hoped, as despite prioritising symptom and peak flow diaries as core components of asthma apps, in reality few patients engage regularly with monitoring tasks60. Future studies will need to explore the feasibility of using digital support for self-...
The IL-23-Th17 axis is responsible for neutrophilic inflammation in various inflammatory diseases. Here, we discover a potential pathway to inhibit neutrophilic asthma. In our neutrophil-dominant asthma (NDA) model, single-cell RNA-seq analysis identifie
Cambridge, UK) (9), have been published. In this study, the 10th, 50th and 90th percentile cohort values were calculated at each time point interval (0.01 s) and the relevant flow curves generated. The two individual profiles exhibiting the lowest and highest peak inspiratory flow (PIF) ...