Methods We have developed a thyroid cancer tumor bank to complement our Oral, Head and Neck Cancer Program. This tumor bank cryopreserves enzymatically isolated viable cells from resected tumors (n = 16).We are also attempting to develop primary cell lines and are isolating and assessing ...
The BLAST 2.0 software (gapped BLAST at the NCBI Internet site) was used for searching for homologies between human PRDX5 (162 amino acids) and known polypeptides in databases (GenBank, SwissProt). Said search did not give perfect alignment with known peptides from different species (Table 1)...
were performed at Unité Etude du Polymorphisme des Génomes Végétaux, INRA, using the Bank service and Illumina sequencers facilities of CEA-Institut de Génomique/CNG, Evry (France). All the DNA samples went through quality control successfully. Non-indexed paired-ends (PE) libraries were ...
The CRC cell line SW620 was purchased from the Korean Cell Line Bank (Seoul, Korea) and maintained in complete RPMI medium (LM011-01; WELGENE Inc., Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS, 16000044; Thermo Fisher Scientific, Waltham, MA) and Antibiotic-Antimycotic ...
(GenBank Accession Number: MN095349) and MZC-20 (Acc. Nº: MN095350) was confirmed asA. flavuswith the reference sequence ofA. flavusCICC 40,186 (Acc. Nº: KX462752; identity = 99.8%); the isolates MZC-14 (Acc. Nº: MN095351) and MZC-18 (Acc. Nº: MN069571) asA. ...
The results were compared in the GenBank database using BLAST software version 2.15.0 available at NCBI [20]. Genus and species assignment was based on the closest e-value to zero, highest bit score, highest coverage and percent identity; sequences were then grouped into operational taxonomic ...