Different skin cancer types comprise both common and tumor-type-specific CAF subsets Subclustering segregated four CAF populations: mCAFs, iCAFs,RGS5+cells, and ucCAFs. Matrix CAFs (mCAFs) exhibited increased expression of extracellular matrix components such as collagens (COL1A1,COL1A2,COL3A1)...
Residual binding sites on the filter were blocked by incubating the membrane in TBS (10 mmol/L Tris-HCl, pH 8.0, 150 mmol/L NaCl) containing 1% gelatin (Bio-Rad Lab, Richmond, CA) for 1 hour at 25°C. The blots were then washed in TBST (TBS with 0.05% Tween 20) and incubated ...
Sanchez GJ, Richmond PA, Bunker EN, Karman SS, Azofeifa J, Garnett AT, et al. Genome-wide dose-dependent inhibition of histone deacetylases studies reveal their roles in enhancer remodeling and suppression of oncogenic super-enhancers. Nucleic Acids Res. 2018;46(4):1756–76. Article CAS Google...
stringent q-value threshold of 0.01 (1% false discovery rate) was set to ensure robust identification of differentially secreted factors. This analysis revealed 9 cytokines that were significantly altered in the LINE-1 + condition compared to NT-iMG: IL-10, RANTES, VEGF-A, IL-1α, MIP-...
A 20 μL reaction containing 100 ng of cDNA, 10 μM of each primer, and 10 μL of iTaq Universal SYBR Green Supermix (Bio-Rad, Richmond, CA, USA) was performed using the following program: 94 °C for 4 min, followed by 40 cycles of 94 °C for 20 s, 56...
(v/v) glycerol at −20 °C. The strains were identified by aligning 16S rRNA gene sequencing, with sequences aligned to the NCBI database and submitted to GenBank (SUB11132737). Detailed information on the probiotic strains is provided in TableS1. Phylogenetic analysis was performed using...
Northwestern University, Feinberg School of Medicine, 250 E Superior, Chicago, IL, 60611, USA Seema Khan Staten Island Hospital, 1408 Richmond Road, Staten Island, NY, 10304, USA Jordan Glaser Duke University Medical Center, 2301 Erwin Road, Durham, NC, 27710, USA Coleen K. Cunningham Contribu...
The presentation of gout and its various stages from acute to chronic is reviewed in detail. Associated dietary and genetic factors and their presentation are reviewed. This chapter forms the basis for the diagnosis and management of gout that are discussed in subsequent chapters. ...
Groom JR, Richmond J, Murooka TT, Sorensen EW, Sung JH, Bankert K, et al. CXCR3 chemokine receptor-ligand interactions in the lymph node optimize CD+ T helper 1 cell differentiation. Immunity. 2012;37(6):1091–103. Article CAS PubMed PubMed Central Google Scholar Griffith JW, Sokol...
Richmond, T.T. Murooka, E.W. Sorensen, J.H. Sung, K. Bankert, U.H. von Andrian, J.J. Moon, T.R. Mempel, A.D. Luster CXCR3 chemokine receptor-ligand interactions in the lymph node optimize CD4+ T helper 1 cell differentiation Immunity, 37 (2012), pp. 1091-1103, 10.1016/j...