69 Sources of heterogeneity were examined by exploratory subgroup analyses for meta-analyses with at least 10 studies included65,70 using the following covariates: country income group, according to the World Bank classification37; physician specialty; geographical region, according to the United Nations...
The crystal structures have been deposited to the RCSB Protein Data Bank. Time-resolved FRET Binding affinities for MCL-1, BCL-2, BCL-XL, BCL-W, and BCL2-A1 were determined using a time-resolved fluorescence resonance energy transfer (FRET) assay. Test compounds were serially diluted in ...
Table 2 GenBank accession numbers for the blood pathogens detected in this study Full size table Results Clinical signs and microscopic examination of thin blood smears Clinical signs such as anorexia, profuse ocular discharge, emaciation, weakness, and dyspnoea were observed in most of the sampled...
Isoprene DNA-SIP incubations and DNA extraction For SIP incubations, leaves (approximately 2.5 m above ground level) were removed from the south-facing side of a willow tree (Salix fragilis), located on the campus of the University of East Anglia. Cells were dislodged from leaves (approx. 5...
(n = 1; Kawuku)) at three separate sites throughout Uganda were confirmed positive for ZIKV RNA by cPCR and Sanger sequencing (123-bp amplicon) (Fig.1,S3-8). Sequences were deposited in Genbank. Sequence alignment suggest that the sfRNA detected is most closely related to Asian ...
analyses using maximum likelihood and Bayesian approaches on an alignment containing 755 characters confirmed that this pathogenic Perkinsea from North American frogs formed a strongly supported clade within the NAG01 that was distant from the other Perkinsea sequences previously published in GenBank (...
(Promega, Madison, USA) from a pure, culture freshly grown in MB 1:2 and was sequenced on an Illumina MiSeq platform, as described elsewhere [26]. The sequence reads were assembled de novo into contigs with the NGen DNA assembly software by DNAStar, Inc., and the contigs underwent ...
Most of the GII.17 NoV sequences in GenBank only include partial VP1 genes, and very few sequences cover ORF1 and ORF2. The first report of GII.17_2012 in GenBank is from Japan in February 2012; thereafter, such sequences in GenBank are mainly from human specimens ...
Cells were treated with PBS or increasing doses of gemcitabine (ranging from 390 nM to 100 µM) for 72 h and analyzed using Cell Titer 96 Aqueous One Solution/MTS (#G3580, Promega, Madison, Wisconsin) on the GloMax Microplate reader (Promega, Madison, Wisconsin). For IF ...
Acacia mangium, a fast-growing tree native to parts of Indonesia, Papua New Guinea and Australia, has been cultivated outside its native environment and introduced into humid tropical lowland regions of Asia, South America and Africa over the last few de