and then they were determined using AlphaEase FC software (version 6.0) (Alpha Innotech, Watertown, MA, USA). 2.21. NF-κB Nuclear Translocation Assay RAW 264.7 macrophages at a density of 2 × 104cells/well were planted in a 24-well plate with 12 mm chamber slides for 24 h. Cultured ...