coli grown at 37 °C there was no observable condensation, while at lower temperatures (33 °C and 18 °C) enriched protein condensates were observed (Fig. 2d and Supplementary Fig. 16). Live imaging of cells grown at 37 °C as the temperature was reduced confirmed the ...
Brown bear The genomes of hibernating bears such as the brown bear provide key information related to metabolic function. During hibernation (about 6 months out of the year), both brown bears and black bears lower their blood flow and heart rate to as low as 10 beats per minute to protect ...
However, a lower number of TALE genes could be detected from the Flye assembly than from the HGAP4 assembly based on the same PacBio data and, hence, this assembly will not be considered in the remainder of this manuscript. For the hybrid assembly of Illumina and PacBio reads, we also ...
2a, b). After localized reconstruction, the resolution of the Env-corresponding portion was improved from 40 Å or lower to 7.4 and 10.4 Å for the E2p and I3-01v9 SApNP immunogens, respectively. The E2p-L4P NP consists of the E2p shell (based on dihydrolipoyl transacetylase, ...
the need for additional Illumina or PacBio reads used for polishing. However, this comes at the potential risk that true deletions within TALEs are corrected by mistake. Hence, we indicate the sites of computational corrections by lower case letters to make them identifiable in downstream analyses...
[7], can produce short reads (25–300 bp) with high coverage (each nucleotide is covered by > 30 different reads) and high error rate using much less time and cost. Theoretically, we can determine the sequence of a genome in much shorter time and lower cost using the NGS technologies....
13. A biomedical device for the distribution or containment of biological substances comprising at least one surface thereof covered with a cured acrylic adhesive composition layer having a wettability equal to or lower than 50°. 14. The biomedical device according to claim 13 wherein said cured...
4 µM CHIR99021 exposure resulted in the highest cardiomyocyte content with 64.9 ± 5.3% TNNT2 + cells at day 15 (Fig. 1d, Supplementary Fig. 1a). We believe enhanced differentiation at lower CHIR concentrations can be attributed to endogenous morphogen production due to the self-...
Moreover, the term “alkyl” (or “lower alkyl”) includes both “unsubstituted alkyls” and “substituted alkyls,” the latter of which refers to alkyl moieties having substituents replacing hydrogen on one or more carbons of the hydrocarbon backbone. Such substituents may include, for example,...
To release the plate 112 in order that it may be restored to vertical position, the operator pushes upon the knob 147a whereupon the plate 112 is released and it descends and causes the rod 143 to strike the lower edge of the lever 140 and cam the same upwardly against the action of ...