Rapid and selective theophylline serum and saliva assay by means of high pressure liquid chromatographyA rapid, selective and sensitive high pressure liquid Chromatographic (HPLC) method for the determination of theophylline in human serum (or plasma) and saliva was developed.When using 0.5 ml of ...
The solid phase and the specific binding substance bind to each other, for example, by chemical means such as a covalent bond, or by adsorption. The equivalent substance may be indirectly immobilized on the solid phase in advance before use. Or the sample is subjected to the measurement while...
Article preview Abstract Introduction Section snippets References (41) Cited by (44) Steroids Volume 72, Issue 8, July 2007, Pages 633-642Fast and easy in vitro screening assay for cholesterol biosynthesis inhibitors in the post-squalene pathway ...
After the extraction, the relevant compound can be desorbed from the fiber and then be analyzed by means of techniques appropriate to that end such as for instance GC or HPLC. One of the properties of SPME is that only the freely dissolved fraction of the compound is available for ...
reaction were extracted with water-saturatedn-butanol and analyzed by HPLC–MS/MS or by a Coulter counter (radiometric method). Values were given as means of three independent experiments performed. All protein concentrations were determined using theBradford protein assay(Bio-Rad, USA) with bovine...
Simultaneous quantitative assay of phenobarbital, phenytoin, and p-hydroxydiphenylhydantoin by HPLC and a comparison with EMIT. The antiepileptic drugs, phenobarbital and phenytoin, were determined in plasma by enzyme immunoassay (EMIT) and by high-pressure liquid chromatography (HP... C Daniel,del Vi...
approach is to complete a simultaneous saccharification and fermentation (SSF) process [3] and to assay residual sugars and inhibitory products such as glucose, cellobiose, and acetic acid by high performance liquid chromatography (HPLC), and ethanol concentration by gas chromatograph (GC) or HPLC....
4A). Specificity means that the result of the experiment is not caused by non-specific interactions between cells in the system and the assay could unequivocally evaluate the analyte of interest in the presence of other components. When we replaced the 293T/MERS with the parental 293T cells ...
assay is carried out by incubating with a sample containing antibodies directed to the peptide fragment to form an antibody peptide complex, separating unreacted antibodies from the complex immobilized onto said solid matrix, and quantitating the amount of antibody in said complex with detecting means....
By the way, in WO 01/22085 described above, a protein having a molecular weight of 36 kDa is exemplified as one of molecular species of the low-molecular-weight CD14 as an analyte of the assay. By the way, the “soluble CD14” described in the present specification means a protein ...