A short DNA segment in the promoter, between nucleotides -268 and -213 upstream of the ATG start codon, is essential for high-level expression of the A. gossypii TEF gene. It carries two sequences, GCCCATACAT and ATCCATACAT, with high homology to the UAS-rpg sequence of S. cerevisiae,...
Remarkably, the toxicity did not stem from the heterologous expression of HIS3 (Schizosaccharomyces pombe his5+), but from the multi-copy introduction of the promoter driving this gene; the EF-1α promoter from Ashbya gossypii. This promoter is included in many ubiquitously used heterologous ...
摘要: DNA sequence suitable as promoter, containing the primary structure shown in SEQ ID NO: 1 positions 9-307.收藏 引用 批量引用 报错 分享 全部来源 求助全文 掌桥科研 相似文献Sequence and promoter analysis of the highly expressed TEF gene of the filamentous fungus Ashbya gossypii...
The native TEF promoter revealedto be the best promoter for the expression of recombinant -galactosidase in A. gossypii,leading to 2 and 7 times more extracellular activity than the GPD promoter and theheterologous promoters, respectively. Furthermore, the levels of recombinant -galactosidase activity...
1994: Sequence and promoter analysis of the highly expressed TEF gene of the filamentous fungus Ashbya gossypii Molecular and general genetics: MGG 242(3): 263-271 Wendland, Jürgen.; Philippsen, P. 2002: An IQGAP-related protein, encoded by AgCYK1, is required for septation in the ...
Promoter from Ashbya gossypiiA DNA sequence containingJose Luis Revuelta DovalMaria Angeles Santos GarciaMarkus PompejusHarald Seulberger
relates to a method suitable for use as a promoter DNA sequence, which comprises: a) from SEQ ID NO: 9-307 primary structure represented by bit 1, or b) under standard conditions with one containing a) double-stranded hybrids and primarily a) a primary structure identical promoter activity....
Steiner S,Philippsen P.Sequence and promoter analysis of the highly expressed TEF gene of the filamentous fungus Ashbya gossypii.Molecular and General Genetics. 1994Steiner, S. and Philippsen, P. (1994). Sequence and promoter analysis of the highly expressed TEF gen...
DNA sequence which is suitable as a promoter, containing a) the primary structure which appears in SEQ ID NO:1 position 9 to 307 or b) a primary structure which hybridises with the double strand containing a) in standard conditions and which has essentially the same promoter activities as a...
Ashbya gossypii promoter.DNA sequence suitable as promoter, containing the primary structure shown in SEQ ID NO: 1 positions 9-307.REVUELTA DOVAL, JOSE LUISSEULBERGER, HARALDSANTOS GARCIA, MARIA ANGELESPOMPEJUS, MARKUS