The replacement of mature miRNA sequence by the new one induces promising molecules into the silencing approaches for plant benefits and improvisation. The gene knockdown into the plants is executed by the amiRNAs and theys have minimal exposure into the animal cells. This review summarizes amiRNAs...
miRNAamiRNARNA silencingPlant expression vectorTarget gene expressionArabidopsisIn plants, double-stranded RNA (dsRNA) is an effective trigger of RNA silencing, and several classes of endogenous small RNA (sRNA), processed from dsRNA substrates by DICER-like (DCL) endonucleases, are essential in ...
artificial miRNAviral resistancecrop protectionRNA silencing is a host defense mechanism against viruses in plants (Ding and Voinnet, 2007). As a counter defense mechanism, viruses have adapted ways to overcome the host RNA silencing pathway, such as viral suppressors of RNA silencing (VSR) (In...
Since mutants mimic loss-of-function phenotypes, siRNA-based gene silencing tools were initially applied in functional genomics and trait modification in plants. However, such prototypes of RNAi technology suffer from widespread off-target silencing. Artificial miRNA-based silencing platform was designed ...
antigens. Their uniquely targeted gene-altering miRNA cargo is also simple for researchers to load because activated exosomes can associate with miRNAs of choice by mere incubation3. Exosomes could therefore be used both to battle pathogens and to facilitate gene therapies...
functional MIRNA precursor (Alvarez et al., 2006; Schwab et al., 2006). AmiRNAs have been used in plants to selec- tively and effectively knockdown reporter and endogenous genes, non-coding RNAs and viruses (Ossowski et al., 2008; Tiwari et al., 2014). Recently, cost- and time-effecti...
et al. Multi-omics analysis of multiple missions to space reveal a theme of lipid dysregulation in mouse liver. Sci. Rep. 9, 19195 (2019). Article Google Scholar Malkani, S. et al. Circulating miRNA spaceflight signature reveals targets for countermeasure development. Cell Rep. 33, 108448 ...
(LettuceGDB) This includes two reference genomes with detailed annotations, resequencing data from over 1 000 lettuce varieties, a collection of more than 1 300 worldwide germplasms and millions of phenotypic records, reanalyses of 256 RNA sequencing datasets, a complete miRNAome, metabolite ...
It should be noted that miRNAs often recognize their target mRNAs using as few as 6–8 nucleotides (the seed region) at their 50 end, with relatively little contribution from the remaining miRNA48. However, we recognize that for many applications in biotechnology and synthetic biology it would ...
This study relates to an amiRNA developed on the sequence of Arabidopsis miRNA319a, targeting the ATP/GTP binding domain of AC1 gene of ToLCNDV. The AC1-amiR was found to regulate the abundance of AC1, providing an excellent strategy in providing defense against ToLCNDV. Transgenic lines ...