(EEC) No 3493/82 of 10 December 1982 on the conclusion of the Agreement in the form of an exchange of letters relating to Article 9 of Protocol 1 to the Agreement between the European Economic Community and the State of Israel and concerning the import into the Community of preserved fruit...
Thus, laser axotomy as described herein is an efficient system for initiating and analyzing the process of regeneration. Protocol 1. Assembling the system The specific components of our system are outlined below. When properly assembled, the user should be able to focus the microscope with one ...
Lung tissues were removed and immunofluorescence staining was performed via a standard protocol. Nonspecific sites were blocked with 3% bovine serum albumin (BSA) in PBS. Anti-S1PR1 (Proteintech Group, Chicago, IL, USA) and anti-CD31 antibodies (Abcam, Cambridge, MA, USA) were used for immunos...
Decision of the Administrative Council of 14 December 2022 amending Article 2 of the Rules relating to Fees and adjusting the amount of the reduction in the fee for the supplementary European search where the international or supplementary international search report was drawn up by one of the Euro...
The protocol involves use of siRNAs to deplete cells of factors of interest, fluorescence imaging of nucleoli in an automated high-throughput platform, and use of dedicated software to determine an index of nucleolar disruption, the iNo score. This scoring system is unique in that it integrates ...
Protocol 1. Preparation of the Crab Stomatogastric Nervous System Adult Cancer pagurus L. were obtained from local sources (Newcastle University, Dove Marine Laboratories) and kept in filtered, aerated seawater (10- 12°C). Animals were anesthetized by packing them in ice for 20- 40 min ...
As a result, this setup allows any laboratory with a reflected light microscope to have access to direct correlative imaging of frozen hydrated samples. Protocol 1. Fabrication and Assembly 1. Place the aluminum heat sink inside the 22.96 cm (9") pie pan approximately 2 cm from the side of...
Wei F, Zeadally S, Vijayakumar P et al (2020) An intelligent terminal based privacy-preserving multi-modal implicit authentication protocol for internet of connected vehicles. IEEE Trans Intell Transp Syst 22(7):3939–3951 Article Google Scholar Zheng Z, Bohong Z (2012) Study on spatial str...
For the invasion assay, the membranes in the upper chambers were coated with 20 μg of Matrigel (Corning, NY, USA) and cultured for 48 h using the protocol described above. Subsequently, the cells that migrated from the upper chambers were fixed with 1% crystal violet staining solution for ...
. The procedure was based on the protocol provided by Invitrogen. The real-time PCR mixture volume was 25 μl including 12.5 μl SYBR Green Mix (Power SYBR® Green PCR Master Mix, Applied Biosystems Inc.), 0.2 μl cDNA, 1 μl primer pair mix (5 pmol/μl each primer...