Polymerase chain reaction (PCR) amplification was performed using a 30-µl reaction mix containing 3 µl of 10× manufacturer’s reaction buffer, 1 µl 10 mM dNTP, 0.5 µl 20-µM primer mix, 1 µl DNA template (between 0.05 and 0.5 µg), 0.5 units Taq DNA polymerase (Ne...
The term ‘promoter’ is a nucleotide sequence which is comprised of consensus sequences which allow the binding of RNA polymerase to the DNA template in a manner such that mRNA production initiates at the normal transcription initiation site for the adjacent structural gene. The expression ‘opera...