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and were used at eight to twelve weeks of age. Mice were Brucella-free and were kept in a Biosafety level three (BSL-3) facility. Studies were performed according to Armed Forces Institute of Pathology Regulation 70-1 and all regulations and guidelines regarding the use of laboratory animals ...
Quantitative real-time PCR (qRT-PCR) is a reliable and reproducible technique for measuring and evaluating changes in gene expression. The most common method for analyzing qRT-PCR data is to normalize mRNA levels of target genes to internal reference gen