Argonaute蛋白的中文名是Ago蛋白(或称作“Argonaute蛋白”)。 Argonaute蛋白的中文解析 Argonaute蛋白的基本介绍 Argonaute蛋白(亦称作“Ago”蛋白)是一类广泛存在于古菌、细菌和真核生物中的高度保守的蛋白。它们由N末端、PAZ、MID和PIWI四个球状结构域组成,以及L1和L2两个连接域。这些结构...
In Trypanosoma brucei, Argonaute1 (TbAGO1) is an essential component of the RNA interference (RNAi) pathway. While characterizing a TbAGO1 conditional knockout cell line, we discovered that, upon blockage of TbAGO1 transcription, the RNAi response to transfected double-stranded RNA was severely ...
Dicer, which contains a PAZ domain (Bernstein et al., 2001b). Several members of the Argonaute family have been identified in genetic screens designed to dissect the RNAi pathway, e.g. in C. elegans (RDE-1 (Parrish and Fire, 2001)), Arabidopsis (AGO1 (Fagard et al., 2000)) and ...
In eukaryotic cells degradation of bulk mRNA in the 5' to 3' direction requires the consecutive action of the decapping complex (consisting of DCP1 and DCP... REHWINKEL,J. - 《Rna-a Publication of the Rna Society》 被引量: 838发表: 2005年 加载更多0...
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(b)核酸酶Argonaute(Ago);和 (c)报告核酸,其中若所述报告核酸被剪切,所述的剪切是可以被检测出的。 2.如权利要求1所述的检测体系,其特征在于,所述的靶标分子是核酸分子,并且所述核酸分子可以和报告核酸是同一种分子; 或者,所述靶标分子是化学小分子,并且所述的检测体系中还包括:(d)别构转录因子,所述的...
The letA (ccdA) and letD (ccdB) genes of the F plasmid, located just outside the sequence essential for replication, contribute to stable maintenance of th... N Murayama,H Shimizu,S Takiguchi,... - 《Journal of Molecular Biology》 被引量: 82发表: 1996年 CSR-1 RNAi pathway positively...
b, Superposition of AGO-clade Argonautes’ structures (hAgo1, PDB: 4kxt; hAgo2, PDB: 4w5n; hAgo3, PDB: 5vm9; hAgo4, PDB: 6oon). c, Superposition of MILI (blue) and hAgo2 (green). d, Superposition of MILI (blue) and TtAgo (light brown) (PDB: 3dlh). Structural differences in...
1b). The target AGO1A expression levels were obviously decreased under K+ deficiency compared with that under normal conditions in JZ34 tomatoes (Fig. 1d). As a target of SlmiR168, SlAGO1A showed a complementary expression pattern, and the expression of SlmiR168 actually responded to the ...
testis lysate increased the rate of target cleavage by affinity-purified MIWI piRISC around 20-fold (Fig.1band Extended Data Fig.2b). This effect cannot be attributed to the lysate contributing additional piRISC, because testis lysate alone did not generate detectable cleavage product (Fig.1b)....