Using cortical neuronal cultures and glutamic acid excitotoxicity and oxygen-glucose deprivation (OGD) stroke models, we demonstrated that poly-arginine and arginine-rich cell-penetrating peptides (CPPs), are h
Arginine-rich cell-penetrating peptides are short cationic peptides capable of traversing the plasma membranes of eukaryotic cells. While successful intracellular delivery of many biologically active macromolecules has been accomplished using these peptides, their mechanisms of cell entry are still under in...
Cell surface proteoglycans (PGs) appear to promote uptake of arginine-rich cell-penetrating peptides (CPPs), but their exact functions are unclear. To address if there is specificity in the interactions of arginines and PGs leading to improved internalization, we used flow cytometry to examine up...
To deliver useful biological payloads into the cytosolic space of cells, cell-penetrating peptides have to cross biological membranes. The molecular features that control or enhance this activity remain unclear. Herein, a dimeric template of the arginine-rich HIV TAT CPP was used to establish the...
Plasmid DNA delivery using fluorescein-labeled arginine-rich peptidesdoi:10.1016/j.bmc.2015.05.025Cell-penetrating peptideArginineGene transferDrug delivery systemArginine (Arg)-rich peptides exhibit an effective cell-penetrating ability and deliver membrane-impermeable compounds into cells. In the present ...
Here, we modified the optical properties of FAM with cationic arginine-rich cell-penetrating peptides (CPPs), tuning the pK<sub>a</sub> value of FAM to adapt well to pH measurement under diverse pH conditions. With increasing length of polyarginine, the pK<sub>a</sub> value of FAM was...
Discussion Arginine-rich peptides or proteins are an important class of biomole- cules that have applications ranging from carriers for genes, drug, and even adhesive molecules, due to their cationic nature, nucleic acid binding ability, and cell-penetrating properties, as well as biocompat- i...
To enhance the cell-penetrating capacity, the PLGA-ASO-NP complex was coated with arginine-rich peptide (ARP) in different N:P molar ratios (PLGA-ASO-NP:ARP = 1: 1, 2: 1, 5: 1). To examine the intracellular and intranuclear delivery, these complexes were treated to fully differentiated...
Arginine-rich cell-penetrating peptides are short cationic peptides capable of traversing the plasma membranes of eukaryotic cells. While successful intracellular delivery of many biologically active macromolecules has been accomplished using these peptides, their mechanisms of cell entry are still under ...
"Delivery of macromolecules using arginine-rich cell-penetrating peptides: ways to overcome endosomal entrapment." AAPS J 11(1): 13-22.EL-SAYED A, FUTAKI S, HARASHIMA H. Delivery of macromolecules using arginine-rich cell-penetrating peptides: ways to overcome endosomal entrapment...